Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1050026 | ENA |
REPOSITORIES: ENA
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Project description:Nutrient starvation is an important survival challenge for bacteria during industrial production of functional foods. Lactobacilli are increasingly being used as probiotics in functional foods. As next-generation sequencing technology has greatly advanced, we performed integrative proteomic and genomic analysis to investigate the response of Lactobacillus casei Zhang to a glucose-restricted environment. L. casei Zhang strains were permitted to evolve in glucose-limited or normal medium from a common ancestor over a 3-year period, and they were sampled after 1000, 2000, 3000, 4000, 5000, 6000, 7000, and 8000 generations and subjected to proteomic and genomic analyses. Genomic resequencing data revealed different point mutations and other mutational events in each generation of L. casei Zhang under glucose limitation stress. The proteins expressed differentially under glucose limitation were found to be significantly related to fructose and mannose metabolism, carbohydrate metabolic processes, lyase activity, and amino acid-transporting ATPase activity. The integrative proteomic and genomic analysis revealed that the mutations protected L. casei Zhang against glucose starvation by regulating other cellular carbohydrate, fatty acid, and amino acid catabolism; phosphoenolpyruvate system pathway activation; glycogen synthesis; ATP consumption; pyruvate metabolism; and general stress response protein expression. The results help reveal the mechanisms of adapting to glucose starvation and provide new strategies for enhancing the industrial utility of L. casei Zhang.
2018-02-08 | PXD006643 | Pride
Project description:The complete mitochondrial genome of Fieberiella septentrionalis (Hemiptera: Cicadellidae: Deltocephalinae)
| PRJNA705294 | ENA
Project description:Fresh tumor tissue from primary endometrial tumors. Technology: L1000 is an array based technology that measured 978 landmark genes that are extrapolated using an algorithm to generate a transcription profile of 12,328 genes. Reference L1000 technology: Subramanian A, et al. A Next Generation Connectivity Map: L1000 Platform And The First 1,000,000 Profiles. Cell. 2017/12/1. 171(6):1437–1452.
2021-07-06 | E-MTAB-10668 | biostudies-arrayexpress