Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative gene expression profiling analysis of scRNA-seq data for MyoD-tdTomato high expressing cultured muscle stem cells from MyoD KI mice

ABSTRACT: Comparative gene expression profiling analysis of scRNA-seq data for MyoD-tdTomato high expressing cultured muscle stem cells from MyoD KI mice

PROVIDER: PRJNA1052701 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27215252_1.fastq.gz	Fastqsanger.gz
	SRR27215252_2.fastq.gz	Fastqsanger.gz
	SRR27215253_1.fastq.gz	Fastqsanger.gz
	SRR27215253_2.fastq.gz	Fastqsanger.gz
	SRR27215254_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Comparative gene expression profiling analysis of scRNA-seq data for MyoD-tdTomato high expressing cultured muscle stem cells from MyoD KI mice

Project description:We previosuly generated MyoD-KI mice to distinguish the heterogenous MuSCs populations based on MYOD expression. Using the mice, we performed scRNA-seq to identify the unique gene expression profile in MyoD-tdTomato high expressing MuSCs.

2024-10-08 | GSE250228 | GEO

Identification of the unique gene expression profile in MyoD-tdTomato Low- and MyoD-tdTomato High-expressing muscle stem cells (MuSCs) populations

Project description:We generated MyoD-KI mice to distinguish the heterogenous MuSCs populations based on MYOD expression. Using the mice, we performed RNA-seq to identify the unique gene expression profile in the heterogenous MuSCs population.

2023-04-04 | GSE211755 | GEO

Identification of the unique gene expression profile in MyoD-tdTomato Low- and MyoD-tdTomato High-expressing muscle stem cells (MuSCs) populations

Project description:Identification of the unique gene expression profile in MyoD-tdTomato Low- and MyoD-tdTomato High-expressing muscle stem cells (MuSCs) populations

| PRJNA872018 | ENA

Transcription profiling of mouse 2C5/7 myoD-/-;myf5-/- fibroblast cells expressing MyoD, Myf5, myogenin, or hrGFP

Project description:Gene expression changes induced by MyoD or Myf5 were examined in a double-knockout fibroblast cell line lacking endogenous functional myoD or myf5 genes. Use of this cell line precluded the possibility of auto- or cross-activation of endogenous myoD or myf5. Myogenin or hrGFP were expressed in parallel samples as controls. Following infection with retrovirus - expressing the relevant myogenic regulatory factor (MRF) from the viral LTR promoter and hrGFP through an IRES element in the same mRNA transcript - GFP+ cells were sorted by FACS and harvested for total RNA. Experiment Overall Design: this experiment include 4 samples and 12 replicates

2008-06-12 | E-GEOD-3245 | biostudies-arrayexpress

Expression data from rhabdomyosarcoma cells expressing Myod and E-protein heterodimer and controls

Project description:Rhabdomyosarcomas (RMS) are characterized by expression of myogenic specification genes, such as MyoD and/or Myf5, as well as their bHLH partners for heterodimerization, the E-proteins. We have shown that expression of a forced heterodimer of MyoD with one of the E2A proteins, E12, leads to differentiation in a RMS cell culture model when exposed to low serum conditions. Keywords: RD expressing Myod~E heterodimers and controls

2009-02-14 | GSE14825 | GEO

Transcription profiling of mouse embryoic fibroblasts expressing wild type MyoD or its non-acetylatable version MyoD RRR to investigate how MyoD acetylation may contribute to differential gene activation.

Project description:The transcription factor MyoD can coax na?e fibroblasts or otherwise committed cells to adopt the skeletal muscle phenotype by activating the muscle gene expression program. Activation of muscle gene expression occurs in quantal steps with not all the target genes of MyoD being activated at the same time. Some genes are induced in the initial phases, others at later stages despite the fact that MyoD is present throughout the differentiation process. MyoD is post-translationally modified by phosphorylation, ubiquitination, and acetylation. Here, we have employed a model system in which MyoD and its non-acetylatable version were inducibly expressed in mouse embryonic fibroblasts derived from mice to investigate how MyoD acetylation may contribute to differential gene activation. Experiment Overall Design: Mouse embryos fibroblasts (MEFs) obtained from MyoD-/-/Myf5-/- animals will be transduced with retroviruses expressing an estrogen receptor hormone binding domain fused to either mouse MyoD wild type (ER-MyoD wt) or MyoD bearing three point mutations at lysine residues 99, 102, and 104 that render it no longer acetylatable ( ER-MyoD RRR). A retrovirus without the ER-MyoD insert was also employed as negative control.</p> Aim 2. We will compare genome-wide expression profiling of MEFs transduced with either ER-MyoD wt or ER-MyoD RRR cultured in the presence of a medium that promotes skeletal muscle differentiation (DM) supplemented with beta-estradiol for 0, 6, 12, and 24 hours, respectively.

2007-11-15 | E-GEOD-6487 | biostudies-arrayexpress

Global proteome of Atg5flox:Slc32a1-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain inhibitory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:Slc32a1-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:Slc332a1-Cretg/wt:tdTomato+ (WT) mice. Neurons in suspension were FACS sorted and inhibitory forebrain neurons expressing tdTomato were forwarded to global proteome analysis assessed by LC-MS/MS.

2022-11-15 | PXD031535 | Pride

Global proteome of Atg5flox:CamKIIα-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain excitatory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:CamKIIα-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:CamKIIα-Cretg/wt:tdTomato+ (WT) mice. Neurons in suspension were FACS sorted and excitatory forebrain neurons expressing tdTomato were forwarded to global proteome analysis assessed by LC-MS/MS.

2022-11-15 | PXD031552 | Pride

MyoD, Myf5, myogenin, or hrGFP expressed in 2C5/7 myoD-/-;myf5-/- fibroblast cells

2005-09-07 | GSE3245 | GEO

Murine colonic epithelial cells: Trabid(Zranb1) C443S hetero knock-in mouse (KI/+) vs. homo knock-in mouse (KI/KI)

Project description:Transcriptional profiling of colonic epithelial cells comparing control Trabid KI/+ with KI/KI

2023-05-24 | GSE204668 | GEO