Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The PAF complex regulates alternative splicing.

ABSTRACT: The PAF complex regulates alternative splicing.

PROVIDER: PRJNA1052729 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24834970_1.fastq.gz	Fastqsanger.gz
	SRR24834970_2.fastq.gz	Fastqsanger.gz
	SRR24834972_1.fastq.gz	Fastqsanger.gz
	SRR24834972_2.fastq.gz	Fastqsanger.gz
	SRR24834973_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

The PAF complex regulates alternative splicing.

Project description:The conserved Eukaryotic PAF complex binds to transcribing RNA polymerase II to control deposition of histone marks during transcription. Recently its role in alternative polyadenylation (selection of mRNA 3’end cleavage sites by CPSF) was described. In this work we show that the PAF complex also regulates alternative splicing.

2024-11-05 | GSE250246 | GEO

An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming

Project description:This SuperSeries is composed of the following subset Series: GSE30995: An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming [RNA-Seq] GSE31006: An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming [ChIP-Seq] GSE31007: An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming [protein binding microarray] GSE31948: An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming [AS microarray] Refer to individual Series

2011-09-14 | E-GEOD-30992 | biostudies-arrayexpress

Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

Project description:Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recombinant Mediator subunit MED23 but not to several other Mediator components. One of these factors, hnRNP L, specifically interacts with MED23 in vitro and in vivo. Consistently, Mediator partially colocalizes with hnRNP L and the splicing machinery in the cell. Functionally Med23 regulates a subset of hnRNP L-targeted alternative splicing (AS) and alternative cleavage and polyadenylation (APA) events as shown by minigene reporters and exon array analysis. ChIP-seq analysis revealed that Med23 can regulate hnRNP L occupancy at their co-regulated genes. Taken together, these results demonstrate a crosstalk between Mediator and the splicing machinery, suggesting a novel mechanism for coupling mRNA processing to transcription. Examination of hnRNP L and H3K36me3 enrichment in sictrl and si23 Hela cells

2012-02-24 | E-GEOD-33887 | biostudies-arrayexpress

Aurora-A phosphorylates splicing factors and regulates alternative splicing

Project description:Aurora-A phosphorylates splicing factors and regulates alternative splicing

| PRJNA674315 | ENA

Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

Project description:Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recombinant Mediator subunit MED23 but not to several other Mediator components. One of these factors, hnRNP L, specifically interacts with MED23 in vitro and in vivo. Consistently, Mediator partially colocalizes with hnRNP L and the splicing machinery in the cell. Functionally Med23 regulates a subset of hnRNP L-targeted alternative splicing (AS) and alternative cleavage and polyadenylation (APA) events as shown by minigene reporters and exon array analysis. ChIP-seq analysis revealed that Med23 can regulate hnRNP L occupancy at their co-regulated genes. Taken together, these results demonstrate a crosstalk between Mediator and the splicing machinery, suggesting a novel mechanism for coupling mRNA processing to transcription. We performed an exon array experiment using HeLa cells expressing Med23, hnRNP L or control siRNAs which were established by a virus-mediated siRNA technology. Each sample was done in three biological replicates. Total RNA of these cell lines was processed and hybridized to the Affymetrix human exon array.

2012-02-24 | E-GEOD-33771 | biostudies-arrayexpress

hnRNPDL extensively regulates transcription and alternative splicing

Project description:hnRNPDL extensively regulates transcription and alternative splicing

| PRJNA428381 | ENA

TfR1 globally regulates transcription and alternative splicing

Project description:TfR1 globally regulates transcription and alternative splicing

| PRJNA544112 | ENA

SETMAR regulates gene expression and alternative splicing

Project description:SETMAR regulates gene expression and alternative splicing

| PRJNA754125 | ENA

Identification of WTAP complex-regulated alternative splicing/polyadenylation

Project description:Identification of WTAP complex-regulated alternative splicing/polyadenylation