Project description:We have mapped regulatory loci for nearly all protein coding genes in the mammalian genome using microarray measurements from a panel of mouse/hamster radiation hybrids. Proof of principle transfection experiment. Pcdh7 (a gene identified as being a regulator of many genes) is transfected into A23 and HEK cells and compared to A23 and HEK cells transfected with an empty vector. Upregulated genes predicted by our approach are compared with the transfection experiments Keywords: tranfection, regulation of gene expression

2008-03-13 | GSE9066 | GEO

Fine mapping of regulatory loci for mammalian gene expression using radiation hybrids.

Project description:We mapped regulatory loci for nearly all protein-coding genes in mammals using comparative genomic hybridization and expression array measurements from a panel of mouse-hamster radiation hybrid cell lines. The large number of breaks in the mouse chromosomes and the dense genotyping of the panel allowed extremely sharp mapping of loci. As the regulatory loci result from extra gene dosage, we call them copy number expression quantitative trait loci, or ceQTLs. The -2log10P support interval for the ceQTLs was <150 kb, containing an average of <2-3 genes. We identified 29,769 trans ceQTLs with -log10P > 4, including 13 hotspots each regulating >100 genes in trans. Further, this work identifies 2,761 trans ceQTLs harboring no known genes, and provides evidence for a mode of gene expression autoregulation specific to the X chromosome.

| S-EPMC3014048 | biostudies-literature

Fine mapping of regulatory loci for mammalian gene expression using radiation hybrids

Project description:This SuperSeries is composed of the SubSeries listed below.

2008-03-13 | GSE9052 | GEO

Mapping regulatory loci protein coding genes IN THE mouse genome USING microarray measurements FROM a panel OF mouse/hamster radiation hybrids (control I)

Project description:We have mapped regulatory loci for nearly all protein coding genes in the mammalian genome using microarray measurements from a panel of mouse/hamster radiation hybrids. Experiment Overall Design: Five replicas of RNA from the A23 cell line vs. itself. These arrays serve as a control to determine the power to detect cis ceQTLs in this experiment.

2008-04-04 | E-GEOD-9040 | biostudies-arrayexpress

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