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Interplay between coding and non-coding regulation drives the Arabidopsis seed-to-seedling transition.

ABSTRACT: Interplay between coding and non-coding regulation drives the Arabidopsis seed-to-seedling transition.

PROVIDER: PRJNA1053369 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27227500_1.fastq.gz	Fastqsanger.gz
	SRR27227500_2.fastq.gz	Fastqsanger.gz
	SRR27227501_1.fastq.gz	Fastqsanger.gz
	SRR27227501_2.fastq.gz	Fastqsanger.gz
	SRR27227502_1.fastq.gz	Fastqsanger.gz

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Interplay between coding and non-coding regulation drives the Arabidopsis seed-to-seedling transition.

Project description:Translation of seed stored mRNAs is essential to trigger germination. However, when RNAPII re-engages RNA synthesis during the seed-to-seedling transition has remained in question. Combining csRNA-seq, ATAC-seq and smFISH in Arabidopsis thaliana we demonstrate that active transcription initiation is detectable during the entire germination process. Features of non-coding regulation such as dynamic changes in chromatin accessible regions, antisense transcription, as well as bidirectional non-coding promoters are widespread throughout the Arabidopsis genome. We show that sensitivity to exogenous ABSCISIC ACID (ABA) during germination depends on proximal promoter accessibility at ABA-responsive genes. Moreover, we provide genetic validation of the existence of divergent transcription in plants. Our results reveal that active enhancer elements are transcribed producing non-coding enhancer RNAs (eRNAs) as widely documented in metazoans. In sum, this study defining the extent and role of coding and non-coding transcription during key stages of germination expands our understanding of transcriptional mechanisms underlying plant developmental transitions.

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Time-series of the seed-to-seedling transition in Arabidopsis thaliana

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Project description:AtSDR4L and DIG1 interact with VAL2 to promote seed-to-seedling transition

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