Project description:Transcriptional profiling of Marchantia polymorpha Takaragaike-1 wild-type genotype, in samples from intact plants (NW, non-wounded) and from wounded plants, both in locally damaged tissue (W, wounded) and in systemic non-wounded tissues of the damaged plants (SD, systemic tissues of damaged plants)
Project description:In order to elucidate the role of the single Marchantia B-GATA ortholog in response to high light intensities, a transcriptomic analysis of Marchantia polymorpha BoGa, Mpb-gata1 mutants and MpB-GATA1ox under high-ligh stress conditions was performed.
Project description:In the present study, Marchantia polymorpha Mppcs loss of function mutants were generated through CRISPR/cas9 mediated genome-editing. To assess whether the knockout of MpPCS gene affects the transcription of M. polymorpha nuclear genes in unstressed condition, the Mppcs-2 knockout mutant and Cam2 wild-type transcriptomes were compared by RNA-Seq.
Project description:Most of our current knowledge about the molecular events ruling plant-virus interaction come from studies focusing on vascular plants. We here characterized the molecular, cellular and physiological events goberning plant-virus interactions in the non-vascular liverwort Marchantia polymorpha.
Project description:The regenerative potential in response to wounding varies largely along species. Within the plant lineage, the liverwort Marchantia polymorpha displays an extraordinary regeneration capacity. However, its molecular pathways controlling the initial regeneration response post wounding is currently unknown. In Arabidopsis, the transcription factor ETHYLENE RESPONSE FACTOR 109 (ERF109) plays an essential role in tissue regeneration. Here, we demonstrate that the Marchantia MpERF15 transcription factor gene represents a functional ortholog of ERF109, being instantly activated following wounding and being essential for gemmaling regeneration following tissue incision. MpERF15 is a downstream target of the MpCOI1 oxylipin receptor, but differently from Arabidopsis ERF109 its wound responsiveness is COI1 independent. At the other hand, and again differently from ERF109, MpERF15 drives oxylipin biosynthesis by controlling the upstream MpAOS2 and MpAOC biosynthesis genes. The resulting rise in the oxylipin dnOPDA levels results in an increase in gemma cell number and apical notch organogenesis, giving rise to highly disorganized and compact thalli. Our data pinpoint MpERF15 as a key factor activating an oxylipin biosynthesis amplification loop following wounding that eventually results in re-activation of cell division and regeneration, and suggest that the genetic networks controlling oxylipin biosynthesis in response to wounding might have been reshuffled over evolution.
Project description:To understand a role of MpMET in maintenance of cytpsine methylation and transposon silencing, we obtained a genome wide profile of 5-methylated cytosine from the Mpmet mutant of a model liverwort, Marchantia polymorpha.
Project description:RNA-seq of Marchantia polymorpha Mpb-gata1 mutants was performed in order to investigate their molecular signature of gene expression changes.