Project description:We investigated the changes in soil microorganisms and their metabolic processes under microplastic pollution by adding different types and concentrations of microplastics (polypropylene plastic, polyethylene terephthalate plastic, polyhexanediol/caprolactone microplastics) to agricultural soil.
Project description:The goal of this study was to evaluate the biological effect of microplastic fibres on nasal epithelium from healthy subjects, as well as asthma and COPD pateients. We demonstrated the distinct biological response of asthmatic and COPD epithelial cells to microplastic fibers stimulation compared to healthy epithelial cells. ANKRD36, BCL2L15, C15orf48, CAPN14, FCGBP, FST, IL-19, MAFF, PGBD5, PKP1 and PTPRH are important markers of epithelial response after microplastic stimulation in obstructive lung disaeses. These mediators are linked to Th2 inflammation, alleviation of stress response, and, most notably, carcinogenesis. We demonstrated the distinct biological response of asthmatic and COPD epithelial cells to microplastic fibers stimulation compared to healthy epithelial cells. ANKRD36, BCL2L15, C15orf48, CAPN14, FCGBP, FST, IL-19, MAFF, PGBD5, PKP1 and PTPRH are important markers of epithelial response after microplastic stimulation in obstructive lung disaeses. These mediators are linked to Th2 inflammation, alleviation of stress response, and, most notably, carcinogenesis. Microplastic stimulation differently modified the response of airway epithelial cells in obstructive lung diseases than in controls. Asthmatic and COPD epithelial cells are more prone to damage after microplastic fibre exposure.
2025-02-12 | GSE273262 | GEO
Project description:Nitrogen Allocation Between Soil and Tomato Plants Under Biodegradable Microplastic Stress
| PRJNA1436761 | ENA
Project description:Transcriptome of Gills in Lateolabrax maculatus under Microplastic Stress
Project description:Nipponbare performs poorly in phosphorus (P) deficient soil whereas a Nipponbare-derived NIL containing the Pup1 allele of donor parent Kasalath is tolerant to P deficiency. In this experiment we compared gene expression patterns in roots of this NIL to Nipponbare, grown either in a P deficient or P fertilized soil. The aim is to separate constitutive differences in expression from those induced by P deficiency. Experiment Overall Design: Total RNA was extrancted from root that were grown under two conditions and prepared three biological replicant in each line (total 6 samples in each line). Experiment Overall Design: We used 6 slides for this experiment, in each slide, Nipponbare and 6-4 samples grown under same condition were hybridized.
Project description:To study whether and how soil nitrogen conditions affect the ecological effects of long-term elevated CO2 on microbial community and soil ecoprocess, here we investigated soil microbial community in a grassland ecosystem subjected to ambient CO2 (aCO2, 368 ppm), elevated CO2 (eCO2, 560 ppm), ambient nitrogen deposition (aN) or elevated nitrogen deposition (eN) treatments for a decade. Under the aN condition, a majority of microbial function genes, as measured by GeoChip 4.0, were increased in relative abundance or remained unchanged by eCO2. Under the eN condition, most of functional genes associated with carbon, nitrogen and sulfur cycling, energy processes, organic remediation and stress responses were decreased or remained unchanged by eCO2, while genes associated with antibiotics and metal resistance were increased. The eCO2 effects on fungi and archaea were largely similar under both nitrogen conditions, but differed substantially for bacteria. Coupling of microbial carbon or nitrogen cycling genes, represented by positive percentage and density of gene interaction in association networks, was higher under the aN condition. In accordance, changes of soil CO2 flux, net N mineralization, ammonification and nitrification was higher under the aN condition. Collectively, these results demonstrated that eCO2 effects are contingent on nitrogen conditions, underscoring the difficulty toward predictive modeling of soil ecosystem and ecoprocesses under future climate scenarios and necessitating more detailed studies.
2015-04-03 | GSE67531 | GEO
Project description:A study of soil microbiome under microplastic addition
Project description:Nipponbare performs poorly in phosphorus (P) deficient soil whereas a Nipponbare-derived NIL containing the Pup1 allele of donor parent Kasalath is tolerant to P deficiency. In this experiment we compared gene expression patterns in roots of this NIL to Nipponbare, grown either in a P deficient or P fertilized soil. The aim is to separate constitutive differences in expression from those induced by P deficiency. Keywords: genotype comparison, constitutive differential expression
Project description:Transcriptome analysis in cotton under drought stress. To study the molecular response of drought stress in cotton under field condition global gene expression analysis was carried out in leaf tissue. Gossypium hirsutum cv. Bikaneri Nerma was used for the gene expression analysis. Cotton plants were subjected to drought stress at peak flowering stage. Leaf samples were collected when the soil moisture content was 19.5% which is 50% of the normal control plots. Gene expression profiles in drought induced and their respective control samples were analyzed using Affymertix cotton Genechip Genome arrays to study the global changes in the expression of genome.
2012-06-04 | GSE29566 | GEO
Project description:Epigenomic changes under cellular stress