Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

METTL3 mediates m6A modification of hsa_circ_0072380 to regulate the progression of gestational diabetes mellitus

ABSTRACT: METTL3 mediates m6A modification of hsa_circ_0072380 to regulate the progression of gestational diabetes mellitus

PROVIDER: PRJNA1053777 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27237100_1.fastq.gz	Fastqsanger.gz
	SRR27237100_2.fastq.gz	Fastqsanger.gz
	SRR27237101_1.fastq.gz	Fastqsanger.gz
	SRR27237101_2.fastq.gz	Fastqsanger.gz
	SRR27237102_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

METTL3 mediates m6A modification of hsa_circ_0072380 to regulate the progression of gestational diabetes mellitus

Project description:M6A modification plays a vital role in gestational diabetes mellitus (GDM) progression. However, the role of METTL3 and differential m6A-modified circRNAs in GDM stay to be investigated. Placental tissue samples from GDM patients and normal controls (NC) were collected to measure changes in m6A modification levels. MeRIP-seq on placental tissue was performed to detect differential m6A-modified circRNAs.High glucose (HG)-treated JEG3 cells were used to establish the GDM cell model. Differentially expressed circRNAs levels in GDM and NC groups was measured by qRT-PCR. We knocked down METTL3 to study its function. Additionally, we conducted functional recovery experiments. Dot blot assay was utilized to assess changes in m6A levels. MeRIP-qPCR was performed to evaluate the effect of knocking down METTL3 on m6A modification of hsa_circ_0072380 in JEG3 cells. Compared with NC group, GDM group exhibited increased levels of m6A modification and METTL3 expression. Differences in m6A modification of circRNAs exist between the GDM and NC groups. Hsa_circ_0000994, hsa_circ_0058733, and hsa_circ_0072380 were significantly down-regulated in GDM group while hsa_circ_0036376, hsa_circ_0000471, and hsa_circ_0001173 showed no significant differences between two groups. HG treatment promoted METTL3 expression and m6A level of JEG3 cells, and inhibited cell proliferation, migration, and invasion abilities. Knocking down METTL3 reversed these effects. After HG treatment, hsa_circ_0072380 was significantly down-regulated. Knocking down METTL3 led to up-regulation of hsa_circ_0072380, while knocking down hsa_circ_0072380 restored the function of SiMETTL3. Additionally, knocking down METTL3 significantly reduced m6A modification of hsa_circ_0072380. METTL3 mediated m6A modification of hsa_circ_0072380 to regulate GDM progression.

2024-12-16 | GSE250374 | GEO

DNA methylation and gestational diabetes mellitus

Project description:DNA methylation and gestational diabetes mellitus

| PRJEB66494 | ENA

RNAseq between gestational diabetes mellitus and healthy control groups

Project description:tsRNA profiles of gestational diabetes mellitus and healthy control groups were generated by deep sequencing using Illumina NextSeq 500.

2024-05-12 | GSE267259 | GEO

Transcriptional profile of peripheral blood mononuclear cells from Gestational Diabetes Mellitus Patients

Project description:The oligo microarray were used to determine gene expression profile of PBMC from gestational diabetes mellitus (GDM) patients.

2012-08-25 | E-MEXP-3349 | biostudies-arrayexpress

Genome wide methylation of cord blood from gestational diabetes mellitus

Project description:Genome wide DNA methylation profiling of cord blood cells obtained from gestational diabetes mellitus (GDM) pregnancies. The Illumina EPIC methylation beadchip array was used to obtain DNA methylation profiles across approximately 850,000 CpG dinucleotide methylation loci in DNA isolated from cord blood. Samples include 165 GDM subjects.

2021-06-23 | GSE122086 | GEO

Transcriptional profiles of microRNAs from peripheral blood mononuclear cells of gestational diabetes mellitus patients

Project description:The oligo micoarrays were used to determine gene expression profiles of peripheral blood mononuclear cells from gestational diabetes mellitus (GDM) patients.

2012-08-23 | E-MEXP-3382 | biostudies-arrayexpress

DIA proteomics for gestational diabetes mellitus

Project description:Gestational diabetes mellitus (GDM), one of the most common pregnancy complications, affects approximately 6% of pregnancies. This study attempted to use the data-independent acquisition (DIA) mass spectrometry (MS) to identify the potential protein biomarkers from the placental tissues from the GDM patients and the normal pregnant women.

2021-12-28 | PXD030656 |

RNAseq between gestational diabetes mellitus and healthy control groups

Project description:RNAseq between gestational diabetes mellitus and healthy control groups

| PRJNA1110765 | ENA

Gestational diabetes mellitus alters placental structure, efficiency, and plasticity

Project description:Gestational diabetes mellitus alters placental structure, efficiency, and plasticity

| PRJNA603282 | ENA

Circulating circRNA signature in pregnancies with gestational diabetes mellitus

Project description:Circulating circRNA signature in pregnancies with gestational diabetes mellitus

| PRJNA757644 | ENA