Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene exprssion profile at single nucleus level of liver tissue from mice infected with dengue virus

ABSTRACT: Gene exprssion profile at single nucleus level of liver tissue from mice infected with dengue virus

PROVIDER: PRJNA1054182 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27277225_1.fastq.gz	Fastqsanger.gz
	SRR27277225_2.fastq.gz	Fastqsanger.gz
	SRR27277226_1.fastq.gz	Fastqsanger.gz
	SRR27277226_2.fastq.gz	Fastqsanger.gz
	SRR27277227_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 62

Similar Datasets

Gene exprssion profile at single nucleus level of liver tissue from mice infected with dengue virus

Project description:Severe dengue fever is associated with different degrees of liver injury. We used single nucleus RNA sequencing (snRNA-seq) to analyze the genetic changes in different cells of liver tissue after dengue virus infection.

2024-05-01 | GSE250505 | GEO

Gene exprssion profile of gestational trophoblastic neoplasms

Project description:Gene exprssion profile of gestational trophoblastic neoplasms

| PRJNA559869 | ENA

Genome-wide analysis of Dengue virus 2 infected cells

Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of Dengue virus 2 and mock infected cells at 24 and 36 hours post infection. Dengue virus NS5 protein plays multiple functions in the cytoplasm of infected cells, enabling viral RNA replication and counteracting host antiviral responses. Here, we demonstrate a novel function of NS5 in the nucleus where it interferes with cellular splicing. Using global proteomic analysis of infected cells together with functional studies, we found that NS5 binds spliceosome complexes and modulates endogenous splicing. In particular, we show that NS5 alone, or in the context of viral infection, interacts with core components of the U5 snRNP particle, CD2BP2 and DDX23, alters the inclusion/exclusion ratio of alternative splicing events, and changes mRNA isoform abundance of known antiviral factors. Interestingly, a genome wide transcriptome analysis, using recently developed bioinformatics tools, revealed an increase of intron retention upon dengue virus infection, and viral replication was improved by silencing specific U5 components. Different mechanistic studies indicate that binding of NS5 to the spliceosome reduces the efficiency of pre-mRNA processing, independently of NS5 enzymatic activities. We propose that NS5 binding to U5 snRNP proteins hijacks the splicing machinery resulting in a less restrictive environment for viral replication. A549 cells where infected with Dengue virus 2 or mock and after 24 and 36 hours post infection mRNA was purified. Then the transcriptional profile of these cells was analyzed using RNA-seq.

2016-08-09 | E-GEOD-84285 | biostudies-arrayexpress

Genome-wide analysis of Dengue virus 2 infected cells

2016-08-09 | GSE84285 | GEO

Gene expression timecourse from Dengue virus infected human endothelial cells

2012-04-11 | GSE34628 | GEO

Gene expression timecourse from Dengue virus infected human endothelial cells

Project description:Dengue viruses cause two severe diseases that alter vascular fluid barrier functions, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). While the mechanisms that lead to vascular permeability are unknown, the endothelium plays a central role in regulating fluid and cellular efflux from capillaries. Thus, dysregulation of endothelial cells functions by dengue virus infection may contribute to pathogenesis and severe disease. We used microarrays to investigate the effect of dengue virus infection on gene expression within primary human endothelial cells at various times post infection and identified numerous upregulated antiviral and immune response genes. Early passage primary endothelial cells (HUVECs) were mock infected (no virus) or infected with dengue virus and total RNA collected at 3 timepoints: 12, 24, and 48 hours post infection. Multiple timepoints were analyzed to identify changes in gene expression levels over time. Gene expression from both mock infected and dengue virus infected endothelial cells was evaluated to determine fold induction at each timepoint.

2012-04-10 | E-GEOD-34628 | biostudies-arrayexpress

Gene expression analysis of dengue-infected cells

Project description:We use RNA-seq on mRNA to identify genes that are differential expressed in human cells following dengue virus infection

2018-12-03 | GSE110512 | GEO

Time course expression data of human cell lines infected with dengue virus

Project description:Microarray data was used to study the gene expression changes following dengue infection at various time points to identify host genes and pathways responsible for dengue virus replication Keywords: time course

2006-11-16 | GSE6048 | GEO

Time course expression data of human cell lines infected with dengue virus serotype2 NGC

2007-03-01 | GSE6239 | GEO

Gene exprssion profile of hepatocellular carcinoma in the radiogenomic analysis

Project description:Gene exprssion profile of hepatocellular carcinoma in the radiogenomic analysis

| PRJNA688923 | ENA