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TLR4 signaling of LPS chemotypes in HEK Blue hTLR4 reporter cells.

ABSTRACT: TLR4 signaling of LPS chemotypes in HEK Blue hTLR4 reporter cells.

PROVIDER: PRJNA1054512 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27291432.fastq.gz	Fastqsanger.gz
	SRR27291433.fastq.gz	Fastqsanger.gz
	SRR27291434.fastq.gz	Fastqsanger.gz
	SRR27291435.fastq.gz	Fastqsanger.gz
	SRR27291436.fastq.gz	Fastqsanger.gz

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TLR4 signaling of LPS chemotypes in HEK Blue hTLR4 reporter cells.

Project description:The discovery of Toll-like receptors (TLRs) represented a significant breakthrough that paved the way for the study of host-pathogen interactions in innate immunity. However, there are still major gaps in understanding TLR function, especially the early dynamics of downstream TLR pathways remains less clear. We characterize a label-free optical biosensor-based assay as a powerful method to detect TLR activation in a native and label-free environment and to delineate the dynamics of TLR pathway activation. To gain a deeper insight into the biological processes underlying the ligand-specific signal traces of different LPS chemotypes in various cell types, RNA-seq analysis of transcriptional changes in HEK Blue hTLR4 reporter cells was performed. After 3 hours of stimulation with LPS E. coli or LPS S. minnesota, HEK Blue hTLR4 reporter cells showed significant changes in RNA expression compared to the unstimulated control.

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TLR4 signaling of LPS chemotypes

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