Project description:CARD14 gene mutations have been shown to be a causitive factor for psoriasis in human patients. CARD14 signalling is poorly understood and very little is known about how it regulates NF-kB activation. Basic research is therefore required to better understand the mechanisms by which is brings about pathology in a gain-of-function state. Analyses of CARD14 mutant versus WT transcription over time in a relevant cell type (keratinocyte) was pursued as one of several means to better understand CARD14-driven psoriasis.
Project description:We propose a tool named TimeReg to do Time Course Regulatory Analysis from paired gene expression and chromatin accessibility data.
Project description:This time course microarray experiment was performed on Saccharomyces cerevisiae to determine the global gene expression alterations due to 3-trifluoromethyl-4-nitrophenol (TFM) exposure over time. In this experiment, yeast grown in standard, glucose-containing media were treated with 0.05mM TFM over a four hour period.