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Transcriptional changes over time in exploring cultures of Streptomyces venezuelae

ABSTRACT: Transcriptional changes over time in exploring cultures of Streptomyces venezuelae

PROVIDER: PRJNA750455 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15283300.fastq.gz	Fastqsanger.gz
	SRR15283302.fastq.gz	Fastqsanger.gz
	SRR15283304.fastq.gz	Fastqsanger.gz
	SRR15283306.fastq.gz	Fastqsanger.gz
	SRR15283307.fastq.gz	Fastqsanger.gz

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Transcriptional changes over time in exploring cultures of Streptomyces venezuelae

Project description:We isolated and sequenced mRNA from Streptomyces venezuelae grown on two different solid media that promote exploratory behaviour in this bacterial species. The data was analyzed using DeSeq2 to identify genes that undergo changes in expression over time as well as differences in gene expression patterns between the two media conditions.

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Transcriptional changes over time in exploring cultures of Streptomyces venezuelae [three growth timepoints]

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Transcriptional changes over time during growth of Streptomyces venezuelae on MYM and MYMG

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Identification by ChIP-Seq of the binding sites of the sigma factor WhiG in the genome of Streptomyces venezuelae

Project description:The WhiG sigma factor gene is required for spore formation is Streptomyces venezuelae. It is similar to the FliA sigma factor of E. coli. WhiG deletion strains are able to make aerial hyphae but are defective in the spore maturation. This ChIP-Seq experiment was carried out to determine all the binding sites WhiG binds to in the genome of Streptomyces venezuelae. Anti-WhiG polyclonal antibodies were used for ChIP-Seq of the wild type (WT) strain after 34 hours of growth in shaken cultures. A WhiG deletion strain was made and anti-WhiG antibodies were used for ChIP-Seq in the deletion strain after 34 hours of growth in shaken cultures. This was used as the negative control and ChIP-Seq peak positions in this were disregarded in the WT.

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Project description:ChIP-chip of genomic DNA of Streptomyces venezuelae using polyclonal antibody against bldN

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Identification by ChIP-Seq of the binding sites of the developmental protein BldC in the genome of Streptomyces venezuelae

Project description:The BldC gene is required for the formation of aerial hyphae Streptomyces venezuelae. It is 68 amino acid DNA-binding protein related to the MerR family of transcription factors. BldC deletion strains are bald because they initiate sporulation prematurely, omitting the formation of aerial hyphae altogether. This ChIP-Seq experiment was carried out to determine all the binding sites BldC binds to in the genome of Streptomyces venezuelae. Anti-BldC antibodies were used for ChIP-Seq of the wild type (WT) strain after 10 and 14 hours of growth in shaken cultures. A BldC deletion strain was made and anti-BldC antibodies were used for ChIP-Seq in the deletion strain after 14 hours of growth in shaken cultures. This was used as the negative control and enrichment in this ChIP-Seq was subtracted from the enrichment in the WT strain.

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