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Robust differentiation of human pluripotent stem cells into mural progenitor cells via transient activation of NKX3.1

ABSTRACT: Robust differentiation of human pluripotent stem cells into mural progenitor cells via transient activation of NKX3.1

PROVIDER: PRJNA1055110 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27307443_1.fastq.gz	Fastqsanger.gz
	SRR27307443_2.fastq.gz	Fastqsanger.gz
	SRR27307444_1.fastq.gz	Fastqsanger.gz
	SRR27307444_2.fastq.gz	Fastqsanger.gz
	SRR27307445_1.fastq.gz	Fastqsanger.gz

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Robust differentiation of human pluripotent stem cells into mural progenitor cells via transient activation of NKX3.1

Project description:Mural cells are central to vascular integrity and function. In this study, we demonstrate the innovative use of the transcription factor NKX3.1 to guide the differentiation of human induced pluripotent stem cells into mural progenitor cells (iMPCs). By transiently activating NKX3.1 in mesodermal intermediates, we developed a method that diverges from traditional growth factor-based differentiation techniques. This approach efficiently generates a robust iMPC population capable of maturing into diverse functional mural cell subtypes, including smooth muscle cells and pericytes. These iMPCs exhibit key mural cell functionalities such as contractility, deposition of extracellular matrix, and the ability to support endothelial cell-mediated vascular network formation in vivo.

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Project description:To investigate the role of NKX3.1 in prostate differentiation, we employed transcriptome analysis of mouse seminal vesicle (from 15-month-old Nkx3.1+/+ mice); mouse prostate (from 4-month-old Nkx3.1+/+ and Nkx3.1-/- mice); human prostate cells (RWPE1 cells engineered with empty vector (altered pTRIPZ), NKX3.1 wild type over-expression, and NKX3.1 (T164A) mutant over-expression); and tissue recombinants (generated from combining engineered mouse epithelial cells (seminal vesicle epithelial cells or prostate epithelial cells from 2-month-old mice) and rat UGS mesenchymal cells). Mouse tissue or human cells were snap frozen for subsequent molecular analysis. This SuperSeries is composed of the SubSeries listed below.

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