Project description:Morchella sextelata, recognized for its medicinal properties and distinctive taste, is a highly valued edible mushroom. Despite its commercial importance, the mushroom's susceptibility to environmental factors and its reactions to herbicides commonly used in cultivation have not been extensively studied. This research aimed to explore how four prevalent herbicides—Trifluralin, Pendimethalin, S-metolachlor, and Acetochlor—affect the mycelial growth of Morchella sextelata. The study involved cultivating the mycelium in a Potato Dextrose Agar (PDA) medium with concentrations of herbicides at ten times the standard application rate, followed by measuring the degree of mycelial growth inhibition. Employing transcriptomic analysis, RNA sequencing was conducted on the mycelium exposed to the herbicides. The subsequent transcriptome profiling indicated that the application of these herbicides impacted various biological pathways within Morchella sextelata, with a notable influence on glycolysis/gluconeogenesis and the glyoxylate cycle, which are crucial for energy production and metabolic processes. While Trifluralin and Pendimethalin demonstrated a less pronounced negative impact on mycelial growth, the study concluded that all tested herbicides were detrimental to Morchella sextelata. It is thus recommended that, in agricultural practice, the use of herbicides be approached with caution, and that application rates be strictly adhered to in order to ensure the healthy cultivation of Morchella sextelata and minimize potential pesticide residue issues.
2024-09-25 | GSE277656 | GEO
Project description:RNA-seq of Morchella importuna at three development growth in mycelium
Project description:We report the effect of incubation temperature on embryos and larvae of Solea senegalensis subjected to two different incubation temperatures (15 °C or 21 °C). We found that at some stages, a higher incubation temperature was associated with the expression of miRNAs positively related with growth
2014-06-09 | GSE58297 | GEO
Project description:Comparative transcriptomics of Morchella importune reveals potential growth genes expression at mycelium into fruiting body formation stage
Project description:In the filamentous fungus Podospora anserina, cell death by incompatibility can be monitored using the conditional self-incompatible (SI) het-R het-V strain. SI strains are homokaryotic strains bearing incompatible het genes in all nuclei. The co-expression of these het genes triggers cell death in all the cells and hence in the entire mycelium. The het-R het-V SI strain, bearing the two incompatible het-R and het-V genes, proved particularly convenient as cell death triggering is thermosensitive (J. Labarère et al., 1973, C R Acad Sci Hebd Seances Acad Sci D, 276) and is induced by a simple shift in growth temperature (from 32°C to 26°C).The development of this cell death reaction has been recorded for 4 h after transfer to the restrictive temperature. A time course (T0h to T4h) of the transcriptomic response to the temperature shift has been explored in parallel within SI (het-R het-V) and wild type (compatible het-r hetV) s strains.
Project description:The transcriptome of Leptosphaeria maculans was analysed in mycelium of the wild type isolate v23.1.3 or in transformants silenced for DIM5 or HP1, two genes encoding enzymes involved in chromatin remodelling. The array probes were designed from gene models from the L. maculans whole genome annotation. The aim of this study was to characterise the effect of chromatin remodelling on gene expression during in vitro growth. We performed 9 hybridizations (NimbleGen) with samples derived from mycelium of a wild type isolate, v23.1.3, of a transformant silenced for HP1 and for a transformant silenced for DIM5. Three replicates each. All samples were labeled with Cy3.
Project description:The transcriptome of Leptosphaeria maculans was analysed in mycelium of the wild type isolate v23.1.3 or in transformants silenced for DIM5 or HP1, two genes encoding enzymes involved in chromatin remodelling. The array probes were designed from gene models from the L. maculans whole genome annotation. The aim of this study was to characterise the effect of chromatin remodelling on gene expression during in vitro growth.
2014-03-06 | GSE50616 | GEO
Project description:Effect of growth temperature on Rhodotorula glutinis ZHK (CK group)
Project description:The effect of factors that affect toxin production i.e. glucose, bicarbonate and growth temperature was investigated by transcriptional profilling using microarrays.
