Project description:Long non-coding RNAs (lncRNA) are transcribed but not translated ribonucleic acids with various functions. We analyzed a so far unreported lncRNAn 342419, which we named MANTIS. A search for micropeptides after overexpression of MANTIS in Human Umbilical Vein Endothelial Cells (HUVEC) with subsequent LC-MS/MS with trypsination showed that none of the micropeptides found had any similarity to potential MANTIS ORFs.
Project description:The angiogenic function of endothelial cells is tightly regulated but the impact of long non-coding RNAs (lncRNA) remains unclear. The lncRNA yielded to be an epigenetically restricted endothelial lncRNAs. Here, the chromatin accessibility after knockdown of MANTIS was investigated. It revealed changes on chromatin accessibility on several promoters which are known to be important for endothelial angiogenic function.
Project description:Long non-coding RNAs (lncRNA) are transcribed but not translated ribonucleic acids with various functions. We analyzed a so far unreported lncRNA n342419, which we named MANTIS. A search for micropeptides after overexpression of MANTIS in Human Umbilical Vein Endothelial Cells (HUVEC) with subsequent LC-MS/MS without trypsination showed that none of the micropeptides found had any similarity to potential MANTIS ORFs, whereas the positive control yielded micropeptides encoded from GFP plasmid.
Project description:Viral siRNA generated from antiviral RNA silencing machinery was profiled using small RNA sequencing from Phalaenopsis orchid mixed infected with CymMV and ORSV
