Project description:This laboratory studies the role of carbohydrates in the development of the central nervous system. Comparison of glycosyltransferase gene expression in developing rodent cerebellum with the aim of understanding the regulation of tissue-specific glycans RNA from adult and postnatal day 7 BALB/c mouse cerebellum was extracted and analyzed for differences in glycosyltransferase expression. Three replicate samples were processed for each age group and hybridized to the GLYCOv2 array.
Project description:Purpose: mRNAs sequencing of the gene expression differences in CCR7-stimulated mDC. The goals of this study are to investigate the biological effect of glycosyltransferase Extl1 in regulating dendritic cell function.
Project description:Activation of murine CD4+ and CD8+ T lymphocytes leads to dramatic remodeling of N-linked glycans. Naïve and activated CD4 T cells, CD8 T cells and B cells were compared for their N-linked glycan structures by MALDI-TOF MS profiling and for expression of glycan transferase genes to assess the biosynthetic basis for any change observed. The major change observed in activated CD4 and CD8 T cells was dramatic reduction of sialylated bi-antennary N-glycans carrying the terminal NeuGc?2-6Gal sequence, and corresponding increase in glycans carrying the Gal?1-3Gal sequence. This change was accounted for by a decrease in the expression of the sialyltransferase ST6Gal, and increase in the expression of the galactosyltransferase ?1-3GalT. Conversely, in B cells no change in terminal sialylation of N-linked glycans was evident, and the expression of the same two glycosyltransferases were increased and decreased, respectively. Keywords = N-linked glycosylation, T cell, B cell, activation, glycosyltransferase, carbohydrate, glycomics, glycan, galactosyltransferase, sialyltransferase Keywords: other
Project description:Purpose: mRNAs sequencing of the gene expression differences in LPS-stimulated Extl1-dificient mDC and wild-type mDC. The goals of this study are to investigate the biological effect of glycosyltransferase Extl1 in regulating dendritic cell function.
Project description:Purpose: mRNAs sequencing of the gene expression differences in CCR7L-stimulated Extl1-dificient mDC and wild-type mDC. The goals of this study are to investigate the biological effect of glycosyltransferase Extl1 in regulating dendritic cell function.
Project description:Investigation of centromeres in the pathogenic yeast Candida parapsilosis, shows that the location of two centromeres are polymorphic within this species. The centromeres consist of large inverted repeats (IRs), surrounding unique sequences. New (neo) centromeres have emerged in one C. parapsilosis isolate even though the original CEN location is undamaged. The neocentromeres do not contain IRs, and have no obvious sequence features.
Project description:This SuperSeries is composed of the SubSeries listed below. The study demonstrate that glycosyltransferase Extl1 plays critical roles in promoting CCR7-mediated DC migration by enhancing CCR7 expression and signaling via HSPG, thus contributing to immune defense against pathogens and prevention of autoimmune diseases.
