Project description:The purpose of this study is to explore the effect of adding Lithospermum purpurea to the diet of soybean meal instead of 50% fish meal on the intestinal health of pearl gentian Grouper through Transcriptome
Project description:Grouper is an important commercial maricultural fish, which suffer viral nervous necrosis (VNN) disease at the larval and juvenile stages, but the changes of transcriptomics and proteomics during viral infection remain unknown. In this study, we applied RNA-seq and label-free mass spectrum for the first time to depict the map of transcriptomics and proteomics in non-infected, susceptible-infected and tolerate-infected grouper in larval stage. Further analyses showed that the transcriptome and proteome change dramatically among 3 distinct groups, indicating that different immune response for infected and perststent grouper in larval stage. These valuable transcriptomics and proteomics datasets enable the investigation of molecular mechanism in nervous necrosis (VNN) virus infection, thus helps the further development of molecular breeding and marine fishery
2019-05-15 | PXD012763 | Pride
Project description:Illumina sequencing of pearl gentian grouper intestine
| PRJNA664416 | ENA
Project description:The transcriptomics in muscle of pearl gentian grouper
| PRJNA833953 | ENA
Project description:PacBio SMRT sequence of Pearl gentian grouper intestine
| PRJNA664623 | ENA
Project description:16s rRNA sequencing in the intestine of pearl gentian grouper
| PRJNA1065578 | ENA
Project description:16s rRNA sequencing in the intestine of pearl gentian grouper
Project description:Plant viruses induce various disease symptoms, but their underlying mechanisms are poorly understood. Kobu-sho is a disease in gentian that show gall formation with ectopic development of lignified cells and vascular tissues such as xylem. Recently, we identified a novel unusual RNA virus, gentian Kobu-sho-associated virus (GKaV) from Kobu-sho-affected gentian. Here, we show that a gene fragment of GKaV, Kobu-sho-inducing factor (KOBU), induces gall formation accompanied with ectopic development of lignified cells and xylem-like tissue in Nicotiana benthamiana. We identify KOBU by screening using potato virus X vector in N. benthamiana, and confirm that transgenic gentian expressing KOBU causes tumorous symptom. Transgenic Arabidopsis thaliana expressing KOBU activates signaling pathways regulating xylem development. KOBU protein forms granules and plate-like structures and co-localizes with mRNA splicing factors within nucleus. Our findings provide novel insights into not only virus disease development but also vascular development in plant.