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Single Cell Chromatin Accessibility Profiling on the mouse kidneys with ischemia-reperfusion injury

ABSTRACT: Single Cell Chromatin Accessibility Profiling on the mouse kidneys with ischemia-reperfusion injury

PROVIDER: PRJNA1057807 | ENA |

REPOSITORIES: ENA

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Single Cell Chromatin Accessibility Profiling on the mouse kidneys with ischemia-reperfusion injury

Project description:We performed single nucleus ATAC (snATAC-seq) to generate cell-type-specific chromatin accessibility atlas of the mouse kidneys with ischemia-reperfusion injury. The single nucleus RNA-seq of the same samples were previously published (GEO139107)

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Transcriptional analysis of murine kidneys after caloric restriction and hypoxic preconditioning in an ischemia-reperfusion injury model

Project description:Preconditioning strategies like caloric restriction (CR) and hypoxic preconditioning (HP) show remarkable protective effects in animal models of acute kidney injury (AKI). Since the underlying molecular effects are still not fully understood we performed an experiment directly comparing CR and HP in a murine model of ischemia-reperfusion injury (IRI) of the kidney. 8 to 12-week-old, male C57BL6/J mice were either put to 4 weeks of caloric restriction (70% of normal food intake) or placed in a hypoxic chamber (8%O2) for 3 consecutive days prior to IRI. Whole kidneys were used for transcriptional analysis (RNAseq) before and after ischemia-reperfusion injury to look for common effects of both modes of preconditioning.

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Transcription profiling of mouse contralateral kidneys in response to ischemia reperfusion injury

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Proteome analysis of membranes from renal ischemia-reperfusion injury mouse models.

Project description:Ischemia-reperfusion injury (IRI) is a well-known model for acute kidney injury (AKI). We applied proteomic analysis to detect membrane proteins from IRI mouse kidneys. The analysis set are composed of negative control (sham operation), samples of 4-hour after IRI, and samples of 8-hour IRI.

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Effects of preoperative fasting on renal ischemia-reperfusion injury in young-lean and aged overweight mice

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