Loropetalum chinense
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ABSTRACT:
PROVIDER: PRJNA1058769 | ENA |
REPOSITORIES: ENA
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Project description:Loropetalum chinense var. rubrum genome
| PRJNA1058446 | ENA
Project description:Flower transcriptome of Loropetalum chinense var. rubrum
| PRJNA1058453 | ENA
Project description:<p><strong>BACKGROUND:</strong> <em>Loropetalum chinense </em>var.<em> rubrum</em> (<em>L. chinense</em> var. <em>rubrum</em>) is a precious, native, coloured-leaf ornamental plant in the Hunan Province. We found a <em>L. chinense</em> var. <em>rubrum</em> tree with 3 different leaf colours: GL (green leaf), ML (mosaic leaf) and PL (purple leaf). The mechanism of leaf coloration in this plant is still unclear. The aim of this study was to identify the metabolites and genes involved in determining the colour composition of <em>L. chinense</em> var. <em>rubrum</em> leaves, using phenotypic/anatomic observations, pigment content detection, and comparative metabolomics and transcriptomics.</p><p><strong>RESULTS:</strong> We observed that the mesophyll cells in PL were purple, those in GL were green and those in ML were a mix of purple-green. The contents of chlorophyll a, b, carotenoids and total chlorophyll in PL and ML were significantly lower than those in GL. While the anthocyanin content in PL and ML was significantly higher than that in GL. The metabolomics results showed that the differences in content of cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, cyanidin 3,5-O-diglucoside, pelargonin and petunidin 3,5-diglucoside in ML, GL and PL were significant. Considering that the trend of anthocyanin content change was consistent with the leaf colour difference, we speculated that these compounds might influence the colour of <em>L. chinense</em> var. <em>rubrum</em> leaves. Finally, using transcriptomics, we identified 9 differentially expressed structural genes (one <em>ANR</em> (<em>ANR1217</em>); four <em>CYP75A</em> (<em>CYP75A1815</em>, <em>CYP75A2846</em>, <em>CYP75A2909</em> and <em>CYP75A1716</em>); four UFGTs (<em>UFGT1876</em>, <em>UFGT1649</em>, <em>UFGT1839</em> and <em>UFGT3273</em>) and nine transcription factors (two <em>MYBs</em> (<em>MYB1057</em> and <em>MYB1211</em>), one <em>MADS-box</em> (<em>MADS1235</em>), two <em>AP2-likes</em> (<em>AP2-like1779</em> and <em>AP2-like2234</em>), one <em>bZIP</em> (<em>bZIP3720</em>), two <em>WD40s</em> (<em>WD2173</em> and <em>WD1867</em>) and one <em>bHLH</em> (<em>bHLH1631</em>) that might be related to flavonoid biosynthesis that impacted the appearance of colour in <em>L. chinense</em> var. <em>rubrum</em> leaves.</p><p><strong>CONCLUSIONS:</strong> This study revealed potential molecular mechanisms associated with leaf coloration in <em>L. chinense</em> var. <em>rubrum</em> by analysing differential metabolites and genes related to the anthocyanin biosynthesis pathway. It also provides a reference for research on leaf colour variation in other ornamental plants.</p>
2023-01-30 | MTBLS5662 | MetaboLights