Spirocerca lupi
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1059675 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:COMPLETE MITOCHONDRIAL GENOME OF ONCHOCERCA LUPI (Nematoda, Onchocercidae)
| PRJNA733160 | ENA
Project description:Wolbachia endosymbiont of Onchocerca lupi isolate:Wolbachia Endosymbiont of Onchocerca lupi Genome sequencing
| PRJNA933935 | ENA
Project description:Onchocerca lupi is a filarial worm parasitizing domestic carnivores and humans. Adult nematodes usually localize beneath in the sclera or in the ocular retrobulbar of infected animals, whilst microfilariae are found in the skin. Therefore, diagnosis of O. lupi is achieved by microscopic and/or molecular detection of microfilariae from skin biopsy and/or surgical removal of adults from ocular tissues of infected hosts. An urgent non-invasive diagnostic tool for the diagnosis of O. lupi in dog is mandatory. In this study, an immunoproteomic analyses was performed using a combination of immunoblotting with O. lupi reactive sera and mass spectrometry. Onchocerca lupi major antigen protein (Ol-MJA) and paramyosin (Ol-PARA) were identified as potential biomarkers for serodiagnosis. Linear epitopes were scanned using high-density peptide microarray. Sera collected from dogs infected with O. lupi and healthy controls led to the identification of 11 immunodominant antigenic peptides (n = 7 for Ol-MJA; n = 4 for Ol-PARA). These peptides were validated using sera of dogs uniquely infected with the most important filarioids infesting dogs either zoonotic (Dirofilaria repens, Dirofilaria immitis) or not (Achontochelonema reconditum and Cercopithifilaria bainae). Six antigenic peptides, three for Ol-MJA and for Ol-PARA, respectively, were selected as potential candidate biomarkers for the serological detection of canine O. lupi infection. The molecular and proteomic dataset herein reported should provide a useful resource for studies on O. lupi toward supporting the development of new interventions (drugs, vaccines and diagnostics) against canine onchocercosis.
2021-09-09 | PXD016311 | Pride
Project description:Coyotes as a reservoir for the emerging zoonotic parasite, Onchocerca lupi, in the southwestern United States
| PRJNA516755 | ENA
Project description:Biting midges (Diptera: Ceratopogonidae) as putative vectors of zoonotic Onchocerca lupi (Nematoda: Onchocercidae) in Northern Arizona and New Mexico, southwestern United States
| PRJNA890670 | ENA
Project description:LupiQuant: A Real-Time PCR based assay for determining host-to-parasite DNA ratios of Onchocerca lupi and host Canis lupus from onchocercosis samples.
| PRJNA802584 | ENA
Project description: Not available
| S-EPMC4672443 | biostudies-literature
Project description:BACKGROUND: Onchocerca lupi is a dog parasite of increasing zoonotic concern, with new human cases diagnosed in Turkey, Tunisia, Iran, and the United States. Information about the morphology of this nematode is scant and a detailed re-description of this species is overdue. In addition, histopathological data of potential usefulness for the identification of O. lupi infections are provided. METHODS: Male and female nematodes, collected from the connective tissue of a dog, were examined using light microscopy and scanning electron microscopy (SEM), and an histological evaluation was performed on biopsy samples from periocular tissues. RESULTS: The morphological identification was confirmed by molecular amplification and partial sequencing of cytochrome oxidase subunit 1 gene. This study provides the first comprehensive morphological and morphometric description of O. lupi from a dog based on light microscopy, SEM, molecular characterization, and histological observations. CONCLUSIONS: Data herein presented contribute to a better understanding of this little known parasitic zoonosis, whose impact on human and animal health is still underestimated. The presence of granulomatous reactions only around the female adult suggests that the release of microfilariae from the uterus might be the cause of the inflammatory reaction observed.
| S-EPMC3818983 | biostudies-literature