Project description:Lambs that inherit a callipyge allele from their dam have an up-regulation of maternally imprinted transcripts near the callipyge mutation but do not exhibit muscle hypertrophy. It is not clear what effects these maternally expressed transcripts have in the muscle or how the inheritance of a maternal callipyge allele prevents the expression of the callipyge phenotype which is seen paternal heterozygotes only. This experiment utilized bovine gene expression arrays to profile the gene expression of the semimembranosus muscle of 30 day old half-sibling lambs of all four possible genotypes with respect to the callipyge allele (+/+, C/+, +/C, and C/C). Experiment Overall Design: 10 lambs were used for this study. N=3 biological reps for C/C genotype, N=3 biological reps for Cmat/+ genotype, N=2 biological reps for +/Cpat genotype, and N=2 biological reps for +/+ genotype. Pairwise contrasts were performed to analyze the presence of a maternally inherited callipyge allele and the effect on a homozygous animal (+/C vs CC; +/+ vs C/+; and C/+ vs C/C). Experiment Overall Design: These data make up two experiments. Experiment Overall Design: [1] The maternal allele (4 genotype) experiment compares all four possible genotypes of a particular allele at one age and one tissue (30 days semimembranosus). Experiment Overall Design: [2] The paternal allele (2 genotype) experiment compares only the NC and the NN genotypes in one tissue (SM) across 4 ages (10, 20, 30, and 80 days). Experiment Overall Design: Images were interpreted with Microarray Suite version 5.0 (MAS5.0) in GCOS with no scaling or normalization (i.e., Samples GSM298143-GSM298164). Images were also interpreted by RMA using Bioconductor package of R (i.e., Samples GSM298165-GSM298186).

2009-06-22 | E-GEOD-11780 | biostudies-arrayexpress

Transcriptome sequencing of Ovis aries

Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1) and performed a detailed survey of gene expression across different tissues. RNA-seq data of 7 tissue types from the reference female Texel and skin tissue from a Gansu alpine fine wool sheep were sequenced.

2014-06-06 | GSE56643 | GEO

Ovis aries

Project description:Longissimus Thoracis and subcutaneous fat transcriptome study in lambs

| PRJNA269055 | ENA

Ovis aries

Project description:Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries

| PRJNA215330 | ENA

Ovis aries

Project description:Comparative analysis of ovine fetal skeletal muscle gene expression: control and intrauterine growth restriction (IUGR)

| PRJNA271349 | ENA

MeDIP sequencing of Ovis aries

Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1). Early-stage Illumina GA sequence platform sequenced less reads in high GC content regions than in other regions. To read through higher GC content regions, we generated 2 Gb MeDIP-seq data for filling gaps in sheep reference genome assembly.

2014-06-06 | E-GEOD-56644 | biostudies-arrayexpress

Ovis aries

Project description:Profiles of gene expression in skeletal muscle in sheep from the middle gestation to postnatal stages

| PRJNA131795 | ENA