Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: glutamic acid addition

PROVIDER: PRJNA1059744 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27425961.fastq.gz	Fastqsanger.gz
	SRR27425962.fastq.gz	Fastqsanger.gz
	SRR27425963.fastq.gz	Fastqsanger.gz
	SRR27425964.fastq.gz	Fastqsanger.gz
	SRR27425965.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Project description:L-Glutamic acid stimulates Apostichopus japonicus

| PRJNA990486 | ENA

Tomato endophytic microbial community shifting by L-glutamic acid

Project description:tomato community shifting by glutamic acid

| PRJEB61093 | ENA

Meta-transcriptomic Analyses Reveal Improved Gamma-amino butyric acid Production Machinery in Levilactobacillus brevis NPS-QW 145 Co-cultured with Streptococcus thermophilus ASCC1275 during Milk Fermentation

Project description:Purpose: High γ-aminobutyric acid (GABA)-producing Levilactobacillus brevis strain NPS-QW 145 along with Streptococcus thermophilus (one of the two starter bacteria used to make yogurt for its proteolytic activity) to enhance GABA production in milk. But a mechanistic understanding on how Levilactobacillus brevis cooperated with S. thermophilus to stimulate GABA production has been lacking. Method: Metatranscriptomic analyses combined with peptidomics were carried out to unravel the casein and lactose utilization patterns during milk fermentation with the co-culture. Results: We found particular peptides hydrolyzed by S. thermophilus 1275 were transported and biodegraded with peptidase in Lb. brevis 145 to meet the growth needs of the latter. In addition, amino acid synthesis and metabolism in Lb. brevis 145 were also activated to further support its growth. Glucose, as a result of lactose hydrolysis by S. thermophilus 1275, but not available lactose in milk, was outcompeted by Lb. brevis 145 as a main carbon source for glycolysis to produce ATP.In the stationary phase, under the acidic condition due to accumulation of lactic acid produced by S. thermophilus 1275, genes expression involved in pyridoxal phosphate (coenzyme of glutamic acid decarboxylase) metabolism and glutamic acid decarboxylase (Gad) in Lb. brevis 145 were induced for GABA production.

2020-09-16 | GSE157976 | GEO

Feierabend2021 - iCGB21FR - GEM of Corynebacterium glutamicum ATCC 13032 (Bielefeld)

Project description:The bacterium Corynebacterium glutamicum can produce ʟ-glutamic acid under certain growth conditions. ʟ-glutamic acid is used as a flavor enhancer, food supplement, or primary chemical raw material. It, therefore, plays an essential economic role with an annual production of over 2½ million tons. Due to metabolic development, the product range of C. glutamicum has been expanded to include all biogenic amino acids, vitamins, and more. Previously published metabolic models of C. glutamicum have been supplemented with new metabolic data and expanded with data from new systems biology programs to result in this consensus model.

2021-10-29 | MODEL2102050001 | BioModels

RNA-seq analysis of isogenic dnaN+ and dnaN-E202K E. coli strains derived from MG1655.

Project description:RNA-seq was performed using 2 isogenic strains of E coli bearing either the wild type dnaN allele (strain VB001), or a dnaN allele with a glutamic acid-202 to lysine substitution (dnaN-E202K; strain VB019).

2021-06-02 | GSE175936 | GEO

Drosophila Embryo Culture Gad1 Expressin Neurons

Project description:Positively sorted Drosophila Embryo Culture Gad1 (glutamic acid decarboxylase 1) expressing neurons. FACS sorted after 3-5 days in culture. Labeled with 2.1 kb Gad1-RFP reporter gene. Keywords = Gad1 Gad Expressing Neurons Motor Keywords: other

2004-07-04 | GSE1048 | GEO

lake water metagenome

Project description:Amino acid addition experiment Metagenome

| PRJNA295905 | ENA

The transcriptome of gadE, hdeA, or hdeD null strains compared to wild-type of E. coli under minimum medium conditions

Project description:To compare transcriptional profiling of a gadE-, hdeA-, or hdeD- deficient strain to wild type in E. coli under minimum medium conditions. These genes are included in the GAD cluster genes, which are involved in the glutamic acid-dependent acid resistance (GAD) in E. coli. Goal was to determine the GadE, HdeA, or HdeD regulon in E. coli. Biological replicates: 2 replicates.

2021-06-27 | GSE178954 | GEO

SuperSAGE analysis of the Nicotiana attenuata transcriptome after wounding

Project description:Fatty acid-amino acid conjugates (FACs) in the oral secretion (OS) of the Manduca sexta larvae are necessary and sufficient to elicit the herbivory-specific responses in Nicotiana attenuata, an annual wild tobacco species. We used SuperSAGE combined with 454 sequencing to quantify the early transcriptional changes elicited by the FAC N-linolenoyl-glutamic acid (18:3-Glu) in N. attenuata. The second fully expanded leaf of rosette stage plants were wounded by rolling a fabric pattern wheel three times on each side of the midvein and the wounds were immediately supplied with either 20 uL of 0.02% (v/v) Tween-20/water (solvent control) or 10 uL of synthetic N-linolenoyl-glutamic acid (18:3-Glu; 0.03 nmol/ÂµL in 0.02% (v/v) Tween-20/water). Tissue was collected after 30 min of the treatments and frozen in liquid nitrogen. Total RNA was extracted by the phenol/chloroform-LiCl method and poly(A)+-RNA was purified from total RNA. Subsequent steps for the construction of the SuperSAGE libraries and 454 sequencing were performed as previously described using double NlaIII digestions (Molina et al., 2008, BMC Genomics, 9:553). Two SuperSAGE libraries were generated after wounding and FAC elicitation of Nicotiana attenuata leaves. Leaves were harvested after 30 min of the treatments, total RNA extracted, polyA+ mRNA isolated and used for cDNA synthesis and SuperSAGE analysis as described my Matsumura et al (2003).

2010-10-05 | E-GEOD-18595 | biostudies-arrayexpress

Lysine 117 Residue is Essential for the Function of the Hepatocyte nuclear factor 1α

Project description:Hepatocyte nuclear factor 1α (HNF1α) play essential roles in controlling development and metabolism, its mutations are clearly linked to the occurrence of maturity-onset diabetes of the young (MODY3) in human. 117 lysine to glutamic acid (E117) mutation in the HNF1α gene was clinically associated with MOYD3, but no functional data on this variant is available. Here, we generated a knock-in mouse model in which the lysine 117 is replaced with glutamic acid in the endogenous murine HNF1α locus. The HNF1αE117/E117 mice reproduced HNF1-null phenotypes including dwarfism, hepatic dysfunction, renal Fanconi syndrome, diabetes and die from a progressive wasting syndrome, but no hyperphenylalaninemia. Moreover, HNF1αE117/E117 mice shared similar hepatic gene expression profiles with HNF1 deficient mice. In vitro experiments showed that HNF1 mutants in which the lysine 117 residue were replaced by glutamic acid, arginine or glutamine residues, respectively, had a significant decreased in overall transactivation and DNA binding capacity. K117 mutants had no effect on its protein stabilities and nuclear localization, but significantly blocked its own association. Our results clearly demonstrate that the lysine 117 residue is essential for the function of the HNF1a, and prove important to identify the molecular basis for diseases associated with defects in HNF1α We then performed gene expression profiling analysis using data obtained from RNA-seq of liver tissue of 3 WT mice and 3 KI mice.

2023-01-01 | GSE207758 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data