Project description:The high-throughput RNA-seq was applied under both 26°C and 30°C heat stress in Apostichopus japonicus, which revealed characterization of gene expression at sub-lethal and lethal limit temperature. The results will help to better understand the characteristics of heat shock response in A. japonicus.
Project description:Sea cucumber, Apostichopus japonicus is a very important species for aquaculture, and its behavior and physiology can respond to the initial change in salinity. It is important to understand the molecular responses of A. japonicus when exposed to ambient changes in salinity In this study, RNA-seq provided a general overview of the gene expression profiles of the intestine of A. japonicus exposed to high salinity (SD40), normal salinity (SD30) and low salinity (SD20) environment.
Project description:The sea cucumber Apostichopus japonicus is a deposit-feeder and vital for marine benthic ecosystems. Hypoxia lasting for several days can lead to massive mortality in A. japonicus. It is important to understand the molecular responses of A. japonicus when exposed to acute changes in DO concentration. In the present study, RNA-seq provided a general overview of the gene expression profiles of the respiratory tree of A. japonicus exposed to dissolved oxygen (DO) of 8 mg/L (DO8), 4 mg/L (DO4) and 2 mg/L (DO2) conditions.
Project description:Intestine of a total of 9 idividuals was sampled. The individuals were collected from 3 groups: Control (C), Heat stress 6h (HS6h) and Heat stress 48h (HS48h).After protein extraction,trypsin digestion and enrichment of Lys-acetylated peptides, the eluted peptides was applied with LC-MS/MS detection and data analysis.Lysine acetylproteome profiling reveals key acetylated proteins involved in heat shock response in the sea cucumber Apostichopus japonicus
