Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis revealed key genes and pathways of nitrate in the prevention of hypoxia-induced small intestinal epithelial injury

ABSTRACT: Transcriptome analysis revealed key genes and pathways of nitrate in the prevention of hypoxia-induced small intestinal epithelial injury

PROVIDER: PRJNA1060302 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27401769_1.fastq.gz	Fastqsanger.gz
	SRR27401769_2.fastq.gz	Fastqsanger.gz
	SRR27401770_1.fastq.gz	Fastqsanger.gz
	SRR27401770_2.fastq.gz	Fastqsanger.gz
	SRR27401771_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Transcriptome analysis revealed key genes and pathways of nitrate in the prevention of hypoxia-induced small intestinal epithelial injury

Project description:Our study helps to understand the transcriptome level changes in small intestines exposed to hypoxia after nitrate supplementation, to find out which genes or signaling pathways nitrate plays a role in by activating or inhibiting them.

2024-01-02 | GSE252373 | GEO

nitrate induction - nlp mutants-Nitrate induction - nlp mutants

Project description:bra-inra09-02_bioen_nitrogen - nitrate induction - nlp mutants - Short term nitrate induction kinetics in wildtype, nlp7-1, nlp7-3 and nlp6nlp7 - 10 days old seedling grown in liquid culture on 3mM nitrate wer starvec for N for 3 days and the kinetic for the resupply of nitrate was studied during a short kinetic (0,5, 10,20 minutes).

2012-12-01 | GSE39261 | GEO

no treatment / nitrate starvation / nitrate induction-Study of the primary nitrate response (PNR) in nlp2 mutant and WT

Project description:ngs2018_11_nlp2-no treatment / nitrate starvation / nitrate induction-Is NLP2 involved in the PNR Nitrate starvation and nitrate induction effects on wild type and mutant nlp2

2023-02-08 | GSE142628 | GEO

Does Dietary Nitrate Supplementation Improve Aerobic Performance

Project description:The purpose of this study is to determine whether dietary nitrate supplementation improves performance in cardiopulmonary exercise testing (CPET).

| 2180461 | ecrin-mdr-crc

Low levels of nitrate rather than high carbon-nitrogen ratios induce Dissimilatory Nitrate Reduction to Ammonia (DNRA) in Geobacter lovleyi SZ.

Project description:N retention in soils can be stimulated by microorganisms carrying out dissimilatory reduction of nitrate to ammonia (DNRA), a respiratory activity that converts nitrate and/or nitrite to ammonia. Geobacter lovleyi has recently being recognized as a key driver of DNRA, providing a model to investigate the environmental signals that promote nitrate ammonification. Here we show that low nitrate concentrations (5mM) induce DNRA in G. lovleyi independently of the concentration of the electron donor, thus challenging the prevailing view that high carbon-to-nitrogen (C/N) ratio triggers this process. The nitrate transcriptome revealed a complex metabolic network of periplasmic (Nap) and cytoplasmic (Nar) nitrate reductase systems for the reduction of nitrate to nitrite. The transcriptome also included a canonical (NrfA-1), two Geobacter-specific nitrite reductases (NrfA-2 and NrfA-3) and a membrane-bound NrfH cytochrome, which electronically connects NrfA to the menaquinone pool. Flagellar motility and chemotaxis proteins were also among the most upregulated genes in the nitrate cultures, consistent with an adaptive response that allows Geobacter cells to sense and access the limited supply of nitrate in anaerobic zones of the soils and sediments. This is the first demonstration of the ability of the bacteria to use DNRA pathway under nitrate limiting conditions independently of the C/N ratio. G. lovleyi provides a model for study DNRA process and it is a good candidate that could contribute in the retention of nitrogen in soils leading to efficient use of nitrogen containing fertilizers and preventing nitrate leaching.

2024-12-31 | GSE164776 | GEO

BHLHE40 is key hypoxia-induced transcriptional repressor in beta cells

Project description:BHLHE40 is key hypoxia-induced transcriptional repressor in beta cells

| PRJNA836763 | ENA

barley responses to nitrate limitation-BarIEy Stress Tolerance

Project description:ngs2020_19_arimnet-barley responses to nitrate limitation-What are the molecular mechanisms taking place in barley under nitrate limitation?-Barley were grown on sand under 0.5 mM nitrate (Low nitrate= LN) or 5 mM nitrate (high nitrate = HN)

2022-01-24 | GSE188216 | GEO

nitrate induction - nlp mutants-Nitrate induction - nlp mutants

2012-12-01 | E-GEOD-39261 | biostudies-arrayexpress

Identification of the H+-K+-ATPase ATP4A as hypoxia-regulated target for acute kidney injury prevention

Project description:Acute kidney injury (AKI) is the most common disease in critically ill patients and it is an important factor in the increase of morbidity and mortality yet the therapeutic strategy for AKI prevention is not established. Hypoxia Inducible Factors (HIFs) have been studied that play an important role during AKI to reduce its severity. Here we investigated to identify the novel therapeutic target of HIF2A during ischemic AKI. We performed microarray with the vechle- or PT2385 (HIF2A specific inhibitor) to find the downstream gene of Hif2a and identified that H+-K+-ATPase Atp4a was a leading candidate of HIF2A targeted gene which was suppressed by HIF2A in the tubular epithelial cell. We further uncovered that pharmacological inhibition of Atp4a or genetic deletion of Atp4a reduced AKI during renal ischemia and reperfusion.

2024-12-23 | GSE284576 | GEO

Effects of two-component system NarQ/P deletion on gene expression of Actinobacillus pleuropneumoniae in nitrate-rich anaerobic growth

Project description:Nitrate metabolism is an adaptation mechanism used by many bacteria for survival under anaerobic environments. As a by-product of inflammation, nitrate is used by the intestinal bacterial pathogens to enable gut infection. However, the responses of bacterial respiratory pathogens to nitrate are less well understood. Actinobacillus pleuropneumoniae is an important bacterial respiratory pathogen of swine. Previous studies have suggested that adaptation of A. pleuropneumoniae to anaerobiosis is important for infection. In this work, A. pleuropneumoniae growth and pathogenesis in response to the nitrate were investigated. Nitrate significantly promoted A. pleuropneumoniae growth under anaerobic condition in vitro and lethality in mice. By using narQ and narP deletion mutants, and single-residue mutation complementary strains of ΔnarQ, the two-component system NarQ/P were confirmed to be critical for nitrate induced growth, with Arg50 in NarQ as an essential functional residue. Transcriptome analysis showed that nitrate up-regulated multiple energy-generating pathways, including nitrate metabolism, mannose and pentose metabolism and glycerolipid metabolism via the regulation of NarQ/P. Furthermore, narQ, narP and its target gene encoding the nitrate reductase Nap contributed to the pathogenicity of A. pleuropneumoniae. The Nap inhibitor tungstate significantly reduced the survival of A. pleuropneumoniae in vivo, suggesting that Nap is a potential drug target. These results give new insights into how the respiratory pathogen A. pleuropneumoniae utilizes alternative electron acceptor nitrate to overcome the hypoxia microenvironment, which can occur in the inflammatory or necrotic infected tissues.

2022-07-29 | GSE207497 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data