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Identification of the H+-K+-ATPase ATP4A as hypoxia-regulated target for acute kidney injury prevention

ABSTRACT: Acute kidney injury (AKI) is the most common disease in critically ill patients and it is an important factor in the increase of morbidity and mortality yet the therapeutic strategy for AKI prevention is not established. Hypoxia Inducible Factors (HIFs) have been studied that play an important role during AKI to reduce its severity. Here we investigated to identify the novel therapeutic target of HIF2A during ischemic AKI. We performed microarray with the vechle- or PT2385 (HIF2A specific inhibitor) to find the downstream gene of Hif2a and identified that H+-K+-ATPase Atp4a was a leading candidate of HIF2A targeted gene which was suppressed by HIF2A in the tubular epithelial cell. We further uncovered that pharmacological inhibition of Atp4a or genetic deletion of Atp4a reduced AKI during renal ischemia and reperfusion.

ORGANISM(S): Mus musculus

PROVIDER: GSE284576 | GEO | 2024/12/23

REPOSITORIES: GEO

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