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Root transcriptional profile of maize seedlings supplied with FePO4 nanoparticles as source of Fe and P.

ABSTRACT: Root transcriptional profile of maize seedlings supplied with FePO4 nanoparticles as source of Fe and P.

PROVIDER: PRJNA1060666 | ENA |

REPOSITORIES: ENA

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Root transcriptional profile of maize seedlings supplied with FePO4 nanoparticles as source of Fe and P.

Project description:FePO4 nanoparticles (NPs) were used as source of Fe and P for the growth of maize seedlings. Through a series of transcriptional profiles comparison of plants treated with FePO4 NPs, bulk FePO4 (non-nanometric ) and Fe-deficient and P-deficient plants, transcripts speciffically modulated by NPs as source of Fe and P were identified.

2025-03-21 | GSE252443 | GEO

Root transcriptional profile of cucumber seedlings supplied with FePO4 nanoparticles as source of Fe and P.

Project description:FePO4 nanoparticles (NPs) were used as source of Fe and P for the growth of cucumber seedlings. Through a series of transcriptional profiles comparison of plants treated with FePO4 NPs, bulk FePO4 (non-nanometric ) and Fe-deficient and P-deficient plants, transcripts speciffically modulated by NPs as source of Fe and P were identified.

2025-03-26 | GSE252553 | GEO

Root transcriptional profile of cucumber seedlings supplied with FePO4 nanoparticles as source of Fe and P.

Project description:Root transcriptional profile of cucumber seedlings supplied with FePO4 nanoparticles as source of Fe and P.

| PRJNA1061164 | ENA

Transcriptomic changes in maize roots in response to Fe deficiency

Project description:Iron deficiency is a yield-limiting factor and a worldwide problem for crop production in many agricultural regions, particularly in aerobic and calcareous soils. Graminaceous species, like maize, improve Fe acquisition through the release of phytosiderophores (PS) into the rhizosphere and the following uptake of Fe(III)-PS complexes through specific transporters. Transcriptional profile obtained by roots 12-d-old maize plants under Fe starvation for 1 week (Fe-deficient; 19-d-old plants) were compared with the transcriptional profile obtained by roots of 12-d-old maize plants grown in a nutrient solution containing 100 μM Fe-EDTA for 1 week (Fe-sufficient; 19-d-old plants).

2017-06-12 | GSE76829 | GEO

Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium

Project description:Low iron (Fe) bioavailability can limit the biosynthesis of Fe-containing proteins, which are especially abundant in photosynthetic organisms, thus negatively affecting global primary productivity. Understanding cellular coping mechanisms under Fe limitation is therefore of great interest. We surveyed the temporal responses of Chlamydomonas (Chlamydomonas reinhardtii) cells transitioning from an Fe-rich to an Fe-free medium to document their short- and long-term adjustments. While slower growth, chlorosis and lower photosynthetic parameters are evident only after one or more days in Fe-free medium, the abundance of some transcripts, such as those for genes encoding transporters and enzymes involved in Fe assimilation, change within minutes, before changes in intracellular Fe content are noticeable, suggestive of a sensitive mechanism for sensing Fe. Promoter reporter constructs indicate a transcriptional component to this immediate primary response. With acetate provided as a source of reduced carbon, transcripts encoding respiratory components are maintained relative to transcripts encoding components of photosynthesis and tetrapyrrole biosynthesis, indicating metabolic prioritization of respiration over photosynthesis. In contrast to the loss of chlorophyll, carotenoid content is maintained under Fe limitation despite a decrease in the transcripts for carotenoid biosynthesis genes, indicating carotenoid stability. These changes occur more slowly, only after the intracellular Fe quota responds, indicating a phased response in Chlamydomonas, involving both primary and secondary responses during acclimation to poor Fe nutrition.

2024-04-04 | GSE263199 | GEO

Root transcriptional profile of wheat seedlings in response to Fe, S and dual (Fe and S) deficiencies.

Project description:The comparison of transcriptional profiles of roots grown under Fe, S and dual deficiency with those obtained for roots of control plants (grown in a complete nutrient solution) allowed identifying the transcripts differentially expressed in response to each deficiency.

2018-07-30 | GSE98634 | GEO

RNA-seq analysis of Arabidopsis roots of Col-0, fls2 and UBQ10::mCitrine-IMA1 in response to +Fe, +Fe+flg22, -Fe and -Fe+flg22

Project description:We reported the flg22-triggered immune responses in roots affect the iron deficiency responses and may link to the function of FLS2 and IMA1 in the root. To identify the underlying mechanism of how the root transcriptome profiles respond to +Fe, +Fe+flg22, -Fe, -Fe+flg22 respectively, and if the flg22 responses is dependent on the function of FLS2 and IMA1 in the root, we performed an mRNA-seq experiments in Col-0, fls2 and UBQ10::mCitrine-IMA1 with different treatments. The differentially expressed genes in response to +Fe, +Fe+flg22, -Fe, -Fe+flg22 were analyzed. It has 36 samples in total, with 3 biogical replicates for each condition and each genotype.

2023-10-09 | GSE213557 | GEO

Root transcriptional profile of tomato Fe-deficient plants in response to supply with natural chelating agents.

Project description:Tomato, a Strategy I model plant for Fe deficiency, is an important economical crop. The transcriptional responses induced by Fe deficiency in tomato roots were previously described (Zamboni et al., 2012). The changes in trascriptome caused by the supply of Fe to plants starved fro 1 week were described in relation to the different nature of chelating agents (Fe-WEHS, Fe-CITRATE and Fe-PS). Transcriptional profile obtained by roots (27-d) of 21-d-old tomato plants starved of iron (0 Î¼M Fe-EDTA) for 1 week and supplied for 1 h with 1 Î¼M of Fe as Fe-WEHS (supply_Fe_WEHS), Fe citrate (supply_Fe_CITRATE) and Fe-PS (supply_Fe_PS). Tomato plants were hydroponically grown in all three case of Fe supply. Three different biological replicates were used for each sample repeating the experiment three times. All samples were obtained pooling roots of six plants (27-d-old).

2016-01-11 | E-GEOD-69419 | biostudies-arrayexpress

Molecular mechanism of Arabidopsis thaliana seedlings in response to phytotoxicity of yttrium oxide nanoparticles

Project description:Due to the wide application of rare earth oxides nanoparticles in different fields, they will inevitably be released into the environment, and their potential toxicity and ecological risks in the environment have become a concern of people. Yttrium oxide nanoparticles are important members of rare earth oxides nanoparticles. The molecular mechanism of its influence on plant growth and development and plant response to them is unclear. In this study, we found that yttrium oxide nanoparticles above 2 mM significantly inhibited the growth of Arabidopsis seedlings. Using the Arabidopsis marker lines reflecting auxin signal, it was found that the treatment of yttrium oxide nanoparticles led to the disorder of auxin signal in root cells: the auxin signal in quiescent center cells and columella stem cells decreased; while the auxin signal in the stele cells was enhanced. In addition, trypan blue staining showed that yttrium oxide nanoparticles caused the death of root cells. Transcriptome sequencing analysis showed that yttrium oxide nanoparticles specifically inhibited the expression of lignin synthesis related genes, activated mitogen-activated protein kinase (MAPK) signaling pathway, and enhanced ethylene and ABA signaling pathways in plants. This study revealed the phytotoxicity of yttrium oxide nanoparticles at the molecular level, and provided a new perspective at the molecular level for plants to respond to rare earth oxide stress.

2022-10-05 | GSE214612 | GEO

Fe-FIT-Diff

Project description:au13-06_fit - Fe-FIT-Diff - FIT (FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) is a regulator of Fe deficiency responses in the root. FIT is a basic helix-loop-helix protein. Here, we investigated the transcriptome changes in response to Fe deficiency (- Fe) versus the control condition (+ Fe) in wild type, the fit-3 loss of function mutant and in FIT overexpression plants.

2015-02-14 | GSE65934 | GEO

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