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Root transcriptional profile of cucumber seedlings supplied with FePO4 nanoparticles as source of Fe and P.

ABSTRACT: Root transcriptional profile of cucumber seedlings supplied with FePO4 nanoparticles as source of Fe and P.

PROVIDER: PRJNA1061164 | ENA |

REPOSITORIES: ENA

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Root transcriptional profile of cucumber seedlings supplied with FePO4 nanoparticles as source of Fe and P.

Project description:FePO4 nanoparticles (NPs) were used as source of Fe and P for the growth of cucumber seedlings. Through a series of transcriptional profiles comparison of plants treated with FePO4 NPs, bulk FePO4 (non-nanometric ) and Fe-deficient and P-deficient plants, transcripts speciffically modulated by NPs as source of Fe and P were identified.

2025-03-26 | GSE252553 | GEO

Root transcriptional profile of maize seedlings supplied with FePO4 nanoparticles as source of Fe and P.

Project description:FePO4 nanoparticles (NPs) were used as source of Fe and P for the growth of maize seedlings. Through a series of transcriptional profiles comparison of plants treated with FePO4 NPs, bulk FePO4 (non-nanometric ) and Fe-deficient and P-deficient plants, transcripts speciffically modulated by NPs as source of Fe and P were identified.

2025-03-21 | GSE252443 | GEO

Root transcriptional profile of maize seedlings supplied with FePO4 nanoparticles as source of Fe and P.

Project description:Root transcriptional profile of maize seedlings supplied with FePO4 nanoparticles as source of Fe and P.

| PRJNA1060666 | ENA

Iron-deficiency-induced changes in wild type, ubc13A and cucumber CsUBC13 overexpressed Arabidopsis roots

Project description:CsUBC13 was identified via proteomics from iron starvation treated Cucumber root. ubc13A is an ABRC seed stock (CS51269). CS851269 was purchased from ABRC and confirmed as homozygous Atubc13A knock-out T-DNA mutant. We generated transgenic arabidopsis with ectopic expression of CsUBC13 gene under control of the cauliflower 35S promotor. Both genotypes and Col-0 were used to investigate the transcriptional response to Iron (Fe) deficiency.

2009-07-07 | GSE16964 | GEO

Melatonin promotes chilling tolerance of cucumber seedlings by regulating antioxidant systems and relieving photoinhibition

Project description:Chilling is a major stress which adversely decreases photosynthesis and leads to oxidative stress in thermophilic plants. The role of melatonin (MT) in stress response of plants, has so far been investigated, while the mechanisms by which MT regulate photosynthesis in chilling-sensitive cucumber under chilling stress remain unclear. In this paper, we reported MT positively regulated chilling tolerance of cucumber. Moreover, MT showed a concentration-dependent manner and 1.0 μmol·L-1 was the optimum concentration, of which the chilling injury index, EL and MDA were the lowest, while growth was the highest among all the treatments. Further studies indicated that MT triggered the activity and expression of the antioxidant enzymes, which in turn decreased ROS accumulation caused by chilling stress. Meanwhile, MT maintained a high photosynthetic carbon assimilation capacity, increased the activity of PSI reaction center, PSII reaction center and electron transfer efficiency. More importantly, the proteome analysis and western-blot data revealed that PSI-associated proteins (PsaD, PsaE, PsaF, PsaH and PsaN), PSII-associated protein D1 as well as Rubisco large subunit and RCA were significantly up-regulated in MT-treated seedlings, compared to H2O-treated seedlings under chilling stress. These results suggest that MT enhances chilling tolerance of cucumber via activating the antioxidant system as well as protein level of key PSI-, PSII-related and carbon assimilation enzymes to decrease ROS accumulation, and finally alleviates the damage to photosynthetic apparatus under chilling stress.

2022-02-15 | PXD029134 | Pride

Organ specific expression in cucumber

Project description:We compared organ specific chloroplast gene expression in cucumber between fully developed leaves (12th counting from the bottom of plant) and growing tips, female flowers, young leaves, young fruits (3-5 cm long). We have also characterized chloroplast gene transcription in cucumber etiolated seedlings in comparison to mature cucumber leaves.

2011-06-30 | E-MEXP-3227 | biostudies-arrayexpress

Root transcriptional profile of wheat seedlings in response to Fe, S and dual (Fe and S) deficiencies.

Project description:The comparison of transcriptional profiles of roots grown under Fe, S and dual deficiency with those obtained for roots of control plants (grown in a complete nutrient solution) allowed identifying the transcripts differentially expressed in response to each deficiency.

2018-07-30 | GSE98634 | GEO

RNA-seq analysis of Arabidopsis roots of Col-0, fls2 and UBQ10::mCitrine-IMA1 in response to +Fe, +Fe+flg22, -Fe and -Fe+flg22

Project description:We reported the flg22-triggered immune responses in roots affect the iron deficiency responses and may link to the function of FLS2 and IMA1 in the root. To identify the underlying mechanism of how the root transcriptome profiles respond to +Fe, +Fe+flg22, -Fe, -Fe+flg22 respectively, and if the flg22 responses is dependent on the function of FLS2 and IMA1 in the root, we performed an mRNA-seq experiments in Col-0, fls2 and UBQ10::mCitrine-IMA1 with different treatments. The differentially expressed genes in response to +Fe, +Fe+flg22, -Fe, -Fe+flg22 were analyzed. It has 36 samples in total, with 3 biogical replicates for each condition and each genotype.

2023-10-09 | GSE213557 | GEO

Root transcriptional profile of tomato Fe-deficient plants in response to supply with natural chelating agents.

Project description:Tomato, a Strategy I model plant for Fe deficiency, is an important economical crop. The transcriptional responses induced by Fe deficiency in tomato roots were previously described (Zamboni et al., 2012). The changes in trascriptome caused by the supply of Fe to plants starved fro 1 week were described in relation to the different nature of chelating agents (Fe-WEHS, Fe-CITRATE and Fe-PS). Transcriptional profile obtained by roots (27-d) of 21-d-old tomato plants starved of iron (0 Î¼M Fe-EDTA) for 1 week and supplied for 1 h with 1 Î¼M of Fe as Fe-WEHS (supply_Fe_WEHS), Fe citrate (supply_Fe_CITRATE) and Fe-PS (supply_Fe_PS). Tomato plants were hydroponically grown in all three case of Fe supply. Three different biological replicates were used for each sample repeating the experiment three times. All samples were obtained pooling roots of six plants (27-d-old).

2016-01-11 | E-GEOD-69419 | biostudies-arrayexpress

Molecular mechanism of Arabidopsis thaliana seedlings in response to phytotoxicity of yttrium oxide nanoparticles

Project description:Due to the wide application of rare earth oxides nanoparticles in different fields, they will inevitably be released into the environment, and their potential toxicity and ecological risks in the environment have become a concern of people. Yttrium oxide nanoparticles are important members of rare earth oxides nanoparticles. The molecular mechanism of its influence on plant growth and development and plant response to them is unclear. In this study, we found that yttrium oxide nanoparticles above 2 mM significantly inhibited the growth of Arabidopsis seedlings. Using the Arabidopsis marker lines reflecting auxin signal, it was found that the treatment of yttrium oxide nanoparticles led to the disorder of auxin signal in root cells: the auxin signal in quiescent center cells and columella stem cells decreased; while the auxin signal in the stele cells was enhanced. In addition, trypan blue staining showed that yttrium oxide nanoparticles caused the death of root cells. Transcriptome sequencing analysis showed that yttrium oxide nanoparticles specifically inhibited the expression of lignin synthesis related genes, activated mitogen-activated protein kinase (MAPK) signaling pathway, and enhanced ethylene and ABA signaling pathways in plants. This study revealed the phytotoxicity of yttrium oxide nanoparticles at the molecular level, and provided a new perspective at the molecular level for plants to respond to rare earth oxide stress.

2022-10-05 | GSE214612 | GEO

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