Project description:Using quantitative proteomics we identified group of synaptic genes with decreased protein synthesis during homeostatic plasticity. To obtain further information about their mRNA levels/sequences (3’UTR) we performed polyA RNAseq. Using EISA analysis of Ribominus RNAseq dataset we could further differentiate between transcriptional respective post-transcriptional dependent alterations in mRNA levels. In addition Ribominus RNAseq from cell body and processes (dendrites, axons) RNA samples showed us local changes in mRNA levels during homeostatic plasticity. At the end, small RNAseq helped us to identify miRNAs that are increased during homeostatic plasticity and might regulate downregulated genes.
Project description:Untargeted LC-MS/MS in positive ionisation mode of extract and fractions of Sandwithia guyanensis bark (collected in French Guyana)
| MSV000081542 | GNPS
Project description:Whole genome sequence of Mycobacterium ulcerans CSURP7741, a French Guyana clinical isolate
