Proteomics

Dataset Information

0

Protein turnover during homeostatic plasticity


ABSTRACT: Using quantitative proteomics we identified group of synaptic genes with decreased protein synthesis during homeostatic plasticity. To obtain further information about their mRNA levels/sequences (3’UTR) we performed polyA RNAseq. Using EISA analysis of Ribominus RNAseq dataset we could further differentiate between transcriptional respective post-transcriptional dependent alterations in mRNA levels. In addition Ribominus RNAseq from cell body and processes (dendrites, axons) RNA samples showed us local changes in mRNA levels during homeostatic plasticity. At the end, small RNAseq helped us to identify miRNAs that are increased during homeostatic plasticity and might regulate downregulated genes.

OTHER RELATED OMICS DATASETS IN: PRJNA321531

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Marek Rajman  

LAB HEAD: Gerhard Schratt

PROVIDER: PXD004150 | Pride | 2017-04-05

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20121006_MK_Marek_01.raw Raw
20121006_MK_Marek_02.raw Raw
20121006_MK_Marek_03.raw Raw
20121006_MK_Marek_04.raw Raw
20121006_MK_Marek_05.raw Raw
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Publications


Synaptic downscaling is a homeostatic mechanism that allows neurons to reduce firing rates during chronically elevated network activity. Although synaptic downscaling is important in neural circuit development and epilepsy, the underlying mechanisms are poorly described. We performed small RNA profiling in picrotoxin (PTX)-treated hippocampal neurons, a model of synaptic downscaling. Thereby, we identified eight microRNAs (miRNAs) that were increased in response to PTX, including miR-129-5p, who  ...[more]

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