Project description:Lung cells have traditionally been considered homogeneous. Utilizing single-cell RNA sequencing (scRNA-seq), we analyzed the diverse responses of lung cells to Klebsiella infection. Furthermore, we compared the differential gene expression between wild-type mice and STAT1-knockout mice.
Project description:We have demonstrated that allergic airway inflammation (induced by an ovalbumin sensitization and aerosol challenge protocol) decreases lung bacterial burden following lung infection with Klebsiella pneumoniae. The goals of this study are to indentify novel targets that are expressed during allergic airway inflammation in this model that contribute to enhanced lung bacterial immunity.
Project description:To define the hedgehog-interacting protein (Hhip) expression domain in adult mouse lung, we conduct single-cell RNAseq of the mouse lung using 10X scRNAseq technique.
Project description:This study investigates the interactions between Influenza A virus (IAV) and lung tissue at the single-cell level using the 10x Genomics single-cell RNA sequencing (scRNAseq) platform. Lung tissue samples from a donor with COPD were infected with IAV, and both infected and uninfected samples were analyzed 24 hours post-infection. Single-cell suspensions from multiple infected and uninfected tissue pieces were pooled and analyzed to identify cell types and their responses. A total of 10 cell types were identified, including various airway epithelial cells and immune cells. Apoptotic T cells, characterized by high mitochondrial DNA content, were excluded from gene expression analyses. The presence of viral mRNA was used to indicate viral transcription activity, although distinguishing between intracellular synthesis and extracellular uptake of viral mRNA remains challenging.
Project description:http://www.sanger.ac.uk/resources/downloads/bacteria/klebsiella-pneumoniae.htmlThese data are part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/