Project description:Analysis of the effect of G-alpha or GPCR mutation on the Arabidopsis Transcriptome Keywords: Comparitive Genome Hybridization The transcriptomes of wild type and mutants (G-alpha and GPCR) were compared in the current study. The study was carried out on four whole genome oligo arrays. The G-alpha mutant and its corresponding wild type variety were compared on a single array by means of a dual channel experiment (Cy3 and Cy5 labeling). The experiment was repeated on a different array as a flip-dye replicate and the data from both the sets analyzed further.
Project description:Using acRIP-seq, we present transcriptome-wide atlases of ac4C in Arabidopsis thaliana and Oryza sativa. Analysis of ac4C distribution reveals ac4C is enriched near translation start sites in rice while near translation start sites and end sites in Arabidopsis. Further analysis shows ac4C contributes to RNA stability, splicing and translation. We then performed NaCNBH3 treatment and RNA-seq to measure C to T mutation and RNC-seq to measure translation efficiency in Arabidopsis.
