Gallus gallus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA106469 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The retina plays an important regulatory role in ocular growth. To screen for new retinal candidate genes that could be involved in the inhibition of ocular growth, we used chick microarrays to analyze the changes in retinal mRNA expression after myopic defocus was imposed by positive lens-wear. Chicks were raised under a 12h-light/12h-dark cycle (light-onset: 8:00 am and light-offset: 8:00 pm) with unrestricted access to water and food. On the day prior to the experiment, velcro rings were glued to the feathers around the eye under diethylether anaesthesia. Four male white leghorn chicks, aged 9 days, wore +6.9D spectacle lenses over both eyes for 24 hours. Four untreated age-matched male chicks from the same batch served as controls. After decapitation of the chicks, the eyes were enucleated and the retina was prepared. The retinae from both eyes of each chick were pooled for RNA isolation. Experiment Overall Design: Four samples each: Retina of both eyes of +6.9 diopter lens-treated chicks (plus_lens), Retina of both eyes of untreated control chicks (control)
2008-11-04 | E-GEOD-11439 | biostudies-arrayexpress
Project description:The retina plays an important regulatory role in ocular growth. To screen for new retinal candidate genes that could be involved in the inhibition of ocular growth, we used chick microarrays to analyze the changes in retinal mRNA expression after myopic defocus was imposed by positive lens-wear. Chicks were raised under a 12h-light/12h-dark cycle (light-onset: 8:00 am and light-offset: 8:00 pm) with unrestricted access to water and food. On the day prior to the experiment, velcro rings were glued to the feathers around the eye under diethylether anaesthesia. Four male white leghorn chicks, aged 9 days, wore +6.9D spectacle lenses over both eyes for 24 hours. Four untreated age-matched male chicks from the same batch served as controls. After decapitation of the chicks, the eyes were enucleated and the retina was prepared. The retinae from both eyes of each chick were pooled for RNA isolation. Keywords: gene expression comparison
2008-08-22 | GSE11439 | GEO
Project description:Image defocus and altered retinal gene expression in chicks
| PRJNA132531 | ENA
Project description:In chicks, the avian homologue of the early growth response protein-1 (ZENK) has been shown to be increased in a special cell type of the retina, the glucagonergic amacrine cells, under conditions that lead to a reduction in eye growth (myopic defocus, recovery of myopia) and decreased under conditions that enhance ocular growth (hyperopic defocus, form-deprivation). The investigation of Egr-1 knock-out mice showed that homozygous knock-out mice with no functional Egr-1 protein developed relative axial myopia at the age of 42 and 56 days, compared to heterozygous- and wildtype Egr-1 knock-out mice. To clarify the role of Egr-1 in the retinal regulation of eye growth, and to get an idea about the biochemical pathways underlying this mechanism, we studied the role of Egr-1 in more detail using Affymetrix microarrays. Experiment Overall Design: Retinal samples of young homozygous Egr-1 knock-out and wildtype mice at the age of 30 days (hm30 and wt30; no difference in axial eye length yet) and 42 days (hm42 and wt42; already a difference in axial eye length of 59 µm) were taken to compare the mRNA expression changes over time between these two genotypes and within the same genotype between the two age groups.
2009-12-14 | E-GEOD-16974 | biostudies-arrayexpress
Project description:In chicks, the avian homologue of the early growth response protein-1 (ZENK) has been shown to be increased in a special cell type of the retina, the glucagonergic amacrine cells, under conditions that lead to a reduction in eye growth (myopic defocus, recovery of myopia) and decreased under conditions that enhance ocular growth (hyperopic defocus, form-deprivation). The investigation of Egr-1 knock-out mice showed that homozygous knock-out mice with no functional Egr-1 protein developed relative axial myopia at the age of 42 and 56 days, compared to heterozygous- and wildtype Egr-1 knock-out mice. To clarify the role of Egr-1 in the retinal regulation of eye growth, and to get an idea about the biochemical pathways underlying this mechanism, we studied the role of Egr-1 in more detail using Affymetrix microarrays.
2009-11-30 | GSE16974 | GEO
Project description:Following laboratory and clinical findings implicating circadian biology in the pathogenesis of myopia (nearsightedness), we examined gene expression in two crucial tissues controlling post-natal refractive development, the retina and choroid. Inducing unilateral visual form deprivation myopia in young chicks, a widely studied and validated model, we isolated retinal and choroidal tissues every 4 hours over a single day from myopic and contralateral control eyes during a period time when myopia progresses rapidly.
2024-07-01 | GSE261232 | GEO
Project description:Microarray analysis of changes in gene expression within the amacrine cell layer of chicks following optical defocus
| PRJNA118299 | ENA
Project description:During postnatal development, the optical geometry of the eye is refined through a process called emmetropization. During eye emmetropization, positive optical defocus inhibits eye growth whereas negative optical defocus accelerates it. Increased exposure to negative optical defocus leads to the development of myopia. Although several studies investigated gene regulatory networks underlying retinal response to optical defocus, temporal changes that occur during ocular response to optical defocus are poorly understood. Here, we performed a genome-wide analysis of the retinal gene regulatory networks underlying optical-defocus-induced myopia using massive parallel RNA sequencing (RNA-seq) in chickens exposed to negative optical defocus for 1, 3 and 6 days. Our analysis revealed large-scale dynamic temporal changes in the retinal signaling pathways involved in the eye’s response to negative optical defocus. We found that different sets of pathways and biological functions were involved in the early, sustained and delayed response to optical defocus causing myopia. These data refine signaling pathways that can be targeted for myopia control and provide a framework for the development of new treatment options for myopia.
2025-05-19 | GSE203555 | GEO
Project description:During postnatal development, the optical geometry of the eye is refined through a process called emmetropization. During eye emmetropization, positive optical defocus inhibits eye growth whereas negative optical defocus accelerates it. Increased exposure to negative optical defocus leads to the development of myopia. Although several studies investigated gene regulatory networks underlying retinal response to optical defocus, temporal changes that occur during ocular response to optical defocus are poorly understood. Here, we performed a genome-wide analysis of the retinal gene regulatory networks underlying optical-defocus-induced myopia using massive parallel RNA sequencing (RNA-seq) in chickens exposed to negative optical defocus for 1, 3 and 6 days. Our analysis revealed large-scale dynamic temporal changes in the retinal signaling pathways involved in the eye’s response to negative optical defocus. We found that different sets of pathways and biological functions were involved in the early, sustained and delayed response to optical defocus causing myopia. These data refine signaling pathways that can be targeted for myopia control and provide a framework for the development of new treatment options for myopia.
2025-05-19 | GSE203619 | GEO
Project description:During postnatal development, the optical geometry of the eye is refined through a process called emmetropization. During eye emmetropization, positive optical defocus inhibits eye growth whereas negative optical defocus accelerates it. Increased exposure to negative optical defocus leads to the development of myopia. Although several studies investigated gene regulatory networks underlying retinal response to optical defocus, temporal changes that occur during ocular response to optical defocus are poorly understood. Here, we performed a genome-wide analysis of the retinal gene regulatory networks underlying optical-defocus-induced myopia using massive parallel RNA sequencing (RNA-seq) in chickens exposed to negative optical defocus for 1, 3 and 6 days. Our analysis revealed large-scale dynamic temporal changes in the retinal signaling pathways involved in the eye’s response to negative optical defocus. We found that different sets of pathways and biological functions were involved in the early, sustained and delayed response to optical defocus causing myopia. These data refine signaling pathways that can be targeted for myopia control and provide a framework for the development of new treatment options for myopia.
2025-05-19 | GSE203604 | GEO