Project description:We performed in vivo CRISPR screens of Toxoplasma targeting genes with hyperLOPIT-unassigened subcellular localisation during mouse infection.
Project description:We performed in vivo CRISPR screen of Toxoplasma targeting metabolism-related genes to identify in vivo fitness genes in WT and Ifngr1-deficient mice.
Project description:We performed in vivo CRISPR screen of Toxoplasma targeting ROP/GRA genes to identify in vivo fitness genes in WT and Ifngr1-deficient mice.
Project description:We performed in vivo CRISPR screen of Toxoplasma targeting endomembrane and nucleus-related genes to identify in vivo fitness genes in WT and Ifngr1-deficient mice.
Project description:This is data for the evaluation of a new way of counting sgRNAs in CRISPR screens using padlock probes and UMIs. It is compared to the typical PCR-based approach. In particular, a dropout screen was performed in MiaPaCa-2 cells using the Human Kinome CRISPR pooled library (Addgene #75314)