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Transcription factor C/EBP⍺ is required for the development of Ly6Chi monocytes but not Ly6Clo monocytes

ABSTRACT: Transcription factor C/EBP⍺ is required for the development of Ly6Chi monocytes but not Ly6Clo monocytes

PROVIDER: PRJNA1069002 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27724202_1.fastq.gz	Fastqsanger.gz
	SRR27724202_2.fastq.gz	Fastqsanger.gz
	SRR27724203_1.fastq.gz	Fastqsanger.gz
	SRR27724203_2.fastq.gz	Fastqsanger.gz
	SRR27724204_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Transcription factor C/EBP⍺ is required for the development of Ly6Chi monocytes but not Ly6Clo monocytes

Project description:Monocytes comprise two major subsets, Ly6Chi classical monocytes and Ly6Clo non-classical monocytes. Notch2 signaling in Ly6Chi monocytes triggers transition to Ly6Clo monocytes, which require Nr4a1, Bcl6, Irf2 and Cebpb. By comparison, less is known about transcriptional requirements for Ly6Chi monocytes. We find transcription factor CCAAT/enhancer-binding protein alpha (C/EBPα) is highly expressed in Ly6Chi monocytes, but down-regulated in Ly6Clo monocytes. A few previous studies described the requirement of C/EBPα in the development of neutrophils and eosinophils. However, role of C/EBPα for in vivo monocyte development has not been understood. We deleted the Cebpa +37 kb enhancer in mice, eliminating hematopoietic expression of C/EBPα, reproducing the expected neutrophil defect. Surprisingly, we also discovered a severe and selective loss of Ly6Chi monocytes, while preserving Ly6Clo monocytes. We find that BM progenitors from Cebpa +37–/– mice rapidly progress through the cMoP stage to develop directly into Ly6Clo monocytes even in the absence of Notch2 signaling. These results identify a previously unrecognized role for C/EBPα in maintaining Ly6Chi monocyte identity.

2024-03-18 | GSE254202 | GEO

Microarray expression data from bone marrow monocytes and myeloid progenitors

Project description:Ly6Clo monocytes are a myeloid subset that specializes in the surveillance of vascular endothelium. Ly6Clo monocytes have been shown to derive from Ly6Chi monocytes. Notch2 signaling has been implicated as a trigger for Ly6Clo monocyte development, but the basis for this effect is unclear. Here, we examined the impact of Notch2 signaling of myeloid progenitors on the development of Ly6Clo monocytes in vitro. Notch2 signaling induced by delta-like ligand 1 (DLL1) efficiently induced the transition of Ly6Chi TremL4– monocytes into Ly6Clo TremL4+ monocytes. We further discovered two additional transcriptional requirements for development of Ly6Clo monocytes. Deletion of Bcl6 from myeloid progenitors abrogated development of Ly6Clo monocyte development. IRF2 was also required for Ly6Clo monocyte development in a cell-intrinsic manner. DLL1-induced in vitro transition into Ly6Clo TremL4+ monocytes required IRF2 but unexpectedly could occur in the absence of Nur77 or Bcl6. These results imply a transcriptional hierarchy for these factors in controlling Ly6Clo monocyte development. This experiment compare RNA expression profiles between nonclassical monocytes and various cell types involved in their development.

2023-07-28 | GSE218503 | GEO

Gene expression analysis of neutrophils, Ym1+Ly6Chi monocytes and Ym1-Ly6Chi monocytes

Project description:mRNA expression profiles between Ym1+Ly6Chi monocytes and Ym1-Ly6Chi monocytes from LPS-treated mice were analyzed by RNA-sequencing

2021-07-02 | GSE118032 | GEO

Gene expression analysis of neutrophils, Ym1+Ly6Chi monocytes and Ym1-Ly6Chi monocytes

Project description:Gene expression analysis of neutrophils, Ym1+Ly6Chi monocytes and Ym1-Ly6Chi monocytes

| PRJNA484126 | ENA

Bcl6, Irf2 and Notch2 promote nonclassical monocyte development [RNA-Seq]

Project description:Ly6Clo monocytes are a myeloid subset that specializes in the surveillance of vascular endothelium. Ly6Clo monocytes have been shown to derive from Ly6Chi monocytes. NOTCH2 signaling has been implicated as a trigger for Ly6Clo monocyte development, but the basis for this effect is unclear. Here, we examined the impact of NOTCH2 signaling of myeloid progenitors on the development of Ly6Clo monocytes in vitro. NOTCH2 signaling induced by delta-like ligand 1 (DLL1) efficiently induced the transition of Ly6Chi TREML4– monocytes into Ly6Clo TREML4+ monocytes. We further discovered two additional transcriptional requirements for development of Ly6Clo monocytes. Deletion of BCL6 from myeloid progenitors abrogated development of Ly6Clo monocytes. IRF2 was also required for Ly6Clo monocyte development in a cell-intrinsic manner. DLL1-induced in vitro transition into Ly6Clo TREML4+ monocytes required IRF2 but unexpectedly could occur in the absence of NUR77 or BCL6. These results imply a transcriptional hierarchy for these factors in controlling Ly6Clo monocyte development.

2023-08-02 | GSE239604 | GEO

Bcl6, Irf2 and Notch2 promote nonclassical monocyte development [RNA-seq]

2023-07-28 | GSE218502 | GEO

Genome-wide analysis of tissue-specific gene expression by Ly6Chi inflammatory monocytes during Toxoplasma gondii infection

Project description:Analysis of Ly6Chi monocytes from small intestine lamina propria (SILP) and blood of day 8 Toxoplasma gondii infected mice at gene expression level. The hypothesis tested in the present study was that Ly6Chi monocytes from SILP have altered expression of regulatory factors to blood monocytes. Results provide important information on the regulatory to effector balance of genes expressed by Ly6Chi monocytes during an acute inflammatory response. Ly6Chi inflammatory monocytes were sorted by FACS from the blood or small intestine lamina propria (SILP) of Toxoplasma gondii infected C57BL/6 mice. Cells were isolated at day 8 after infection and total RNA obtained from sorted populations. Three biological replicates were acquired for both blood and SILP from pooled animals.

2013-05-25 | E-GEOD-46685 | biostudies-arrayexpress

Genome-wide analysis of tissue-specific gene expression by Ly6Chi inflammatory monocytes during Toxoplasma gondii infection

2013-05-25 | GSE46685 | GEO

Phenotyping of macrophages derived from TpMos and Mature Ly6Chi monocytes

Project description:Monocytes are integral cells of the innate immune system that play important roles in host defence and tissue repair. It is well established that monocytes are derived from the common monocyte progenitor (cMoP) in the bone marrow (BM) before giving rise to Ly6Chi mature monocytes that enter the circulation. However, our lab has discovered that BM Ly6Chi monocytes are not homogenous, and consist of two subpopulations (CXCR4hi and CXCR4lo) in both mice and humans. In particular, the CXCR4hi subset (termed transitional pre-monocyte (TpMo) population) actively proliferates in the BM and matures into the CXCR4lo subset, which represents mature Ly6Chi monocytes. Nevertheless, how this TpMo population may behave beyond their precursor role in the BM remains undefined. While TpMos have been shown to be strictly immobilized in the BM in the steady state, our preliminary data revealed the mobilization of TpMos into the circulation during sepsis. Furthermore, TpMos were found to give rise to more macrophages than mature Ly6Chi monocytes. Our data hence suggests a critical peripheral role for TpMos that may implicate the outcome of sepsis and possible potential implications in future therapies associated with monocytes. Based on our preliminary findings, we aim to have a better understanding of these monocyte subsets and how they are transcriptionally distinct when they have become macrophages. In particular, we would like to determine if TpMo-derived macrophages are less inflammatory compared to mature monocyte-derived macrophages.

2022-03-09 | GSE192449 | GEO

RNA-sequencing analysis of Ly6Chi macrophage and Ly6Clo macrophage harvested from infarcted heart

Project description:C57BL/6 J mice were subjected to ligation of the left anterior descending coronary artery. Ly6Chi macrophages and Ly6Clo macrophages were collected from infarcted hearts at 3 days after MI.

2020-09-27 | GSE115151 | GEO

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