Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Strain engineering for de novo synthesis of sedoheptulose from glucose

ABSTRACT: Strain engineering for de novo synthesis of sedoheptulose from glucose

PROVIDER: PRJNA1070997 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27780802_1.fastq.gz	Fastqsanger.gz
	SRR27780802_2.fastq.gz	Fastqsanger.gz
	SRR27780803.fastq.gz	Fastqsanger.gz
	SRR27780804.fastq.gz	Fastqsanger.gz
	SRR27780805_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Strain engineering for de novo synthesis of sedoheptulose from glucose

Project description:We propose a strain engineering approach for synthesizing sedoheptulose using glucose as sole feedstock. The gene pfkA encoding 6-phosphofructokinase in Corynebacterium glutamicum was inactivated to direct the carbon flux towards pentose phosphate pathway in the cellular metabolic network. This genetic modification successfully enabled the synthesis of sedoheptulose from glucose. Additionally, we identified key enzymes responsible for product formation through transcriptome analysis, and their corresponding genes were overexpressed, resulting in a further 20% increase in sedoheptulose production.

2024-05-31 | GSE254540 | GEO

De novo gene synthesis by an antiviral reverse transcriptase

Project description:De novo gene synthesis by an antiviral reverse transcriptase

| PRJNA1125358 | ENA

De Novo Purine Synthesis Mediates Circadian Control of Cell Cycle in Zebrafish (array)

Project description:Cell cycle and metabolism are two major outputs controlled by circadian rhythm in many organisms. Here we show that the three processes were linked through inosine 5'-phosphate dehydrogenase (impdh), a rate-limiting enzyme in de novo purine synthesis. We using adult zebrafish as a model system,we applied a genome-wide transcriptome approach that allowed us to investigate circadian gene expression. The whole-genome transcriptome profiles of adult brain in time-series were assayed on Agilent zebrafish microarrays. We used a similar statistical method to identify zebrafish circadian genes (ZCOG) as our previous study in larval zebrafish. Three isoforms of impdh show strong circadian oscillations in different tissues of zebrafish. impdh1a contributes to the ocular development and pigment synthesis, impdh2 promotes and impdh1b delays the development. By limiting the GTP required by DNA synthesis, impdh2 contributes to the daily rhythm of S phase in cell cycle. Multiple enzymes in the de novo purine synthesis pathway show the same circadian oscillations with peaks similar to impdh2. The circadian expression of this pathway is conserved in mouse liver. In summary, we show that the circadian regulation of de novo purine synthesis that supplies crucial building blocks for DNA replication is critical for gating cell cycle in circadian rhythm. Adult zebrafish were sacrificed and dissected at 4h intervals starting at CT0 in both LD and DD conditions for 12 time points. Total RNA of individual zebrafish brain was extracted using Trizol (Invitrogen, Carlsbad, CA) according to the manufacturerM-bM-^@M-^Ys instruction. Microarrays were manufactured by Agilent Technologies (Agilent Technologies, Palo Alto, CA), containing 43,603 probes for zebrafish whole-genome transcriptional profiling.

2013-10-01 | E-GEOD-51277 | biostudies-arrayexpress

mTORC1 couples SREBP-dependent transcription and splicing of de novo lipid synthesis enzymes via FAM120A de novo lipid synthesis enzymes via FAM120A

Project description:mTORC1 couples SREBP-dependent transcription and splicing of de novo lipid synthesis enzymes via FAM120A de novo lipid synthesis enzymes via FAM120A

| PRJNA854056 | ENA

De Novo Purine Synthesis Mediates Circadian Control of Cell Cycle in Zebrafish (array)

2013-10-01 | GSE51277 | GEO

Changes in tryptophan de novo NAD synthesis associated with chronic intestinal inflammation

Project description:Tryptophan metabolism and de novo NAD biosynthesis is associated with behavioural changes in IBD patients. The microbiota and tryptophan de novo syntheses of the distal colon, distal colon and brain of C57BL/6 control mice and Winnie mice littermates with chronic intestinal inflammation were compared using RNA-Seq. Differentially expressed genes were analysed using Micromon software. Changes in tryptophan and nicotinamide metabolism-associated gene expressions, metabolites, and abundance of gut microbiota associated with chronic intestinal inflammation and dysregulated tryptophan metabolism in the Winnie mouse distal colon and brain were apparent. Our findings shed light on the physiological alterations in tryptophan metabolism, specifically its diversion from the serotonergic pathway towards the kynurenine pathway and the consequential effects on de novo NAD+ synthesis, in the context of chronic intestinal inflammation

2024-06-28 | GSE264317 | GEO

De novo gene synthesis by an antiviral reverse transcriptase [RIP-seq]

Project description:De novo gene synthesis by an antiviral reverse transcriptase [RIP-seq]

| PRJNA1125610 | ENA

De novo gene synthesis by an antiviral reverse transcriptase [RNA-seq]

Project description:De novo gene synthesis by an antiviral reverse transcriptase [RNA-seq]

| PRJNA1125609 | ENA

De novo gene synthesis by an antiviral reverse transcriptase [cDIP-seq]

Project description:De novo gene synthesis by an antiviral reverse transcriptase [cDIP-seq]

| PRJNA1125611 | ENA

Changes in tryptophan de novo NAD synthesis associated with chronic intestinal inflammation

Project description:Changes in tryptophan de novo NAD synthesis associated with chronic intestinal inflammation