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RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_SMANTIS_RUNX1_KO]

ABSTRACT: RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_SMANTIS_RUNX1_KO]

PROVIDER: PRJNA1071501 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27865948.fastq.gz	Fastqsanger.gz
	SRR27865949.fastq.gz	Fastqsanger.gz
	SRR27865950.fastq.gz	Fastqsanger.gz
	SRR27865951.fastq.gz	Fastqsanger.gz
	SRR27865952.fastq.gz	Fastqsanger.gz

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RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_diff_OCL]

Project description:Monocytes are important for acute myeloid leukemia (AML), but the influence of long non-coding RNAs (lncRNAs) in this context is unknown. Previously we demonstrated that lncRNA SMANTIS maintains endothelial angiogenic functions and endothelial-monocyte adhesion. Here, the role of SMANTIS in monocytes was examined. SMANTIS was higher expressed in monocytes than in endothelial cells, lost in macrophages, and varied in patients with different AML subtypes. Interaction studies revealed that SMANTIS binds RUNX1, a transcription factor frequently mutated in AML, primarily through its Alu-element. RNA-seq after CRISPR/Cas9-mediated deletion of SMANTIS or RUNX1 revealed an association with cell adhesion and both limited the monocyte adhesion to endothelial cells. Mechanistically, SMANTIS KO reduced RUNX1 genomic binding, and interfered the interaction of RUNX1 with EP300. Collectively, SMANTIS interacts with RUNX1 and limits cell adhesion, which may thus offer a novel therapeutic strategy for AML.

2024-08-05 | GSE254681 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_iPSC_diff_Mono]

2024-08-05 | GSE254680 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_SMANTIS_RUNX1_KO]

2024-08-05 | GSE254679 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [CUT&RUN]

2024-08-05 | GSE254674 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [ATAC-seq]

2024-08-05 | GSE254673 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_iPSC_diff_Mono]

Project description:RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_iPSC_diff_Mono]

| PRJNA1071500 | ENA

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_diff_OCL]

Project description:RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_diff_OCL]

| PRJNA1071522 | ENA

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [CUT&RUN]

Project description:RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [CUT&RUN]

| PRJNA1071504 | ENA

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [ATAC-seq]

Project description:RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [ATAC-seq]

| PRJNA1071505 | ENA

Interacting proteins on lncRNA SMANTIS in THP-1 cells

Project description:The lncRNA SMANTIS (formerly known as MANTIS, AK125871, n342419) functions as a regulator of cell function in endothelial cells and for adhesion to monocytes. Here, we investigated the function of SMANTIS in THP-1 monocytes. By using an antisense oligonucleotide pulldown approach followed by LC/MS, the protein interaction partners of SMANTIS in THP-1 cells were identified. This approach was performed in NTC (non-targeting control) THP-1, where SMANTIS is present, and in SMANTIS-LentiCRISPR KO THP-1, where SMANTIS was removed. The data revealed that a lot of RNA-binding proteins interact with SMANTIS.

2025-01-07 | PXD048847 | Pride

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