Proteomics

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Interacting proteins on lncRNA SMANTIS in THP-1 cells

ABSTRACT: The lncRNA SMANTIS (formerly known as MANTIS, AK125871, n342419) functions as a regulator of cell function in endothelial cells and for adhesion to monocytes. Here, we investigated the function of SMANTIS in THP-1 monocytes. By using an antisense oligonucleotide pulldown approach followed by LC/MS, the protein interaction partners of SMANTIS in THP-1 cells were identified. This approach was performed in NTC (non-targeting control) THP-1, where SMANTIS is present, and in SMANTIS-LentiCRISPR KO THP-1, where SMANTIS was removed. The data revealed that a lot of RNA-binding proteins interact with SMANTIS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Cell Culture

SUBMITTER: Ilka Wittig  

LAB HEAD: Matthias S. Leisegang

PROVIDER: PXD048847 | Pride | 2025-01-07

REPOSITORIES: pride

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P21_025_Bea_Mantis_KO_Solvent_MTS_n1_B.raw Raw
P21_025_Bea_Mantis_KO_Solvent_MTS_n1_C.raw Raw
P21_025_Bea_Mantis_KO_Solvent_MTS_n1_D.raw Raw
P21_025_Bea_Mantis_KO_Solvent_MTS_n1_E.raw Raw
P21_025_Bea_Mantis_KO_Solvent_MTS_n2_B.raw Raw
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Publications

Monocytes, the circulating macrophage precursors, contribute to diseases like atherosclerosis and asthma. Long non-coding RNAs (lncRNAs) have been shown to modulate the phenotype and inflammatory capacity of monocytes. We previously discovered the lncRNA SMANTIS, which contributes to cellular phenotype expression by controlling BRG1 in mesenchymal cells. Here, we report that SMANTIS is particularly highly expressed in monocytes and lost during differentiation into macrophages. Moreover, differen  ...[more]

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