Project description:Arthrobacter chlorophenolicus A6 is a 4-chlorophenol degrading soil bacterium with high phyllosphere colonization capacity. Till now the genetic basis for the phyllosphere competency of Arthrobacter or other pollutant-degrading bacteria is uncertain. We investigated global gene expression profile of A. chlorophenolicus grown in the phyllosphere of common bean (Phaseolus vulgaris) compared to growth on agar surfaces. We designed transcriptome arrays and investigated which genes had different transcript levels in the phyllosphere of common bean (Phaseolus vulgaris) as compared to agar surfaces. Since water availability is considered an important factor in phyllosphere survival and activity, we included both high and low relative humidity treatments for the phyllosphere-grown cells. In addition, we determined the expression profile under pollutant exposure by the inclusion of two agar surface treatments, i.e. with and without 4-chlorophenol.

2013-06-21 | E-GEOD-48198 | biostudies-arrayexpress

Soil Earthworm Phyllosphere

Project description:Soil Earthworm Phyllosphere Genome sequencing

| PRJNA1044938 | ENA

soil and phyllosphere

Project description:soil and phyllosphere Raw sequence reads

| PRJNA849277 | ENA

Litter and soil

Project description:Litter and soil Raw sequence reads

| PRJNA950199 | ENA

Microbial community functioning during plant litter decomposition

Project description:<p>Microbial life in soil is fueled by dissolved organic matter (DOM) that leaches from the litter layer. It is well known that decomposer communities adapt to the available litter source, but it remains unclear if they functionally compete or synergistically address different litter types. Therefore, we decomposed beech, oak, pine and grass litter from two geologically distinct sites in a lab-scale decomposition experiment. We performed a correlative network analysis on the results of direct infusion HR-MS DOM analysis and cross-validated functional predictions from 16S rRNA gene amplicon sequencing and with DOM and metaproteomic analyses. Here we show that many functions are redundantly distributed within decomposer communities and that their relative expression is rapidly optimized to address litter-specific properties. However, community changes are likely forced by antagonistic mechanisms as we identified several natural antibiotics in DOM. As a consequence, the decomposer community is specializing towards the litter source and the state of decomposition (community divergence) but showing similar litter metabolomes (metabolome convergence). Our multi-omics-based results highlight that DOM not only fuels microbial life, but it additionally holds meta-metabolomic information on the functioning of ecosystems.</p>

2024-02-14 | MTBLS1885 | MetaboLights

soil and phyllosphere samples

Project description:soil and phyllosphere samples Raw sequence reads

| PRJNA471945 | ENA

Soil, faeces and phyllosphere

Project description:Soil, faeces and phyllosphere Raw sequence reads

| PRJNA392724 | ENA

phyllosphere and soil sample

Project description:phyllosphere and soil sample Raw sequence reads

| PRJNA487669 | ENA