Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR27866006_1.fastq.gz | Fastqsanger.gz | |||
| SRR27866006_2.fastq.gz | Fastqsanger.gz | |||
| SRR27866007_1.fastq.gz | Fastqsanger.gz | |||
| SRR27866007_2.fastq.gz | Fastqsanger.gz | |||
| SRR27866008_1.fastq.gz | Fastqsanger.gz |
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Project description:Intervention type:DRUG. Intervention1:Huaier, Dose form:GRANULES, Route of administration:ORAL, intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary. Control intervention1:None.
Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes.
For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.. Timepoint:RNA sequencing of 240 blood samples of 80 cases and its analysis, scheduled from June 30, 2022..
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Project description:Oxidative stress is a widespread causative agent of disease. Together with its general relevance for biomedicine, such dynamic is recognizably detrimental to space exploration. Among other solutions, cerium oxide nanoparticles (or nanoceria, NC) display a long-lasting, self-renewable antioxidant activity. In a previous experiment, we evaluated oxidative imbalance in rat myoblasts in space – aboard the International Space Station –, and unveiled possible protective effects from NC through RNA sequencing. Here we focus on myoblast response to NC on land by means of proteomics, defining a list of proteins that putatively react to NC and confirming nucleosomes/histones as likely mediators of its molecular action.
2025-06-09 | PXD047391 | Pride
Project description:Primary objectives: Characterization of the macrophage population subset that is modulated by enteric neurons
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Project description:Purpose: Sequencing analysis of plasma cell-free RNA was performed to study the effects of space flight and microgravity environment in mice. Methods: Plasma samples were collected during the JAXA MHU-1 mission as reported previously (Shiba et al., 2017, Scientific Reports). Total RNA was purified from plasma samples and processed for sequencing analysis, to compare RNA expression profiles in ground control, space flight and artificial 1-G control groups.
2022-11-16 | GSE213808 | GEO
Project description:Mouse of SiO2 group were instilled with silica suspension, and mouse of control group were instilled with NS. The mouse mouse were raised for 56 d, and then the ECM was harvest. We found out the main protein deposited in fabrotic lung ECM. By spatial transcriptomics, we analyzed the distribution pattern of transcripts in space. Using sc-RNA sequencing, we investigated the cell resource of IgA.
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Project description:SILAC labeled human kidney cells (293 cells) or bat kidney cells (PakiT03cells)were infected with Hendra virus for 8 or 24 hours and compared to uninfected control cells. Protein identification and quantitation relied on a combination of Uniprot lists of proteins and Proteomics Informed by Transcriptomics (PIT) analysis whereby RNA extracted from the same samples was deep sequenced and the sequencing data was used to construct mRNA from which possible ORFS were inferred and used as a search space by MaxQuant.
2014-11-05 | PXD001165 | Pride
Project description:SILAC labeled human kidney cells (293 cells) or bat kidney cells (PakiT03cells)were infected with Hendra virus for 8 or 24 hours and compared to uninfected control cells. Protein identification and quantitation relied on a combination of Uniprot lists of proteins and Proteomics Informed by Transcriptomics (PIT) analysis whereby RNA extracted from the same samples was deep sequenced and the sequencing data was used to construct mRNA from which possible ORFS were inferred and used as a search space by MaxQuant.
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Project description:Space radiation induces distinct senescent phenotypes: Implications for space travel [bulk RNA-Seq]
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Project description:As Earth’s magnetic field and ozone continue to weaken, space radiation begins pose a significant threat to the health of not only space travelers, but the world’s population. Space radiation and its high-energy and high-charge ions create distinct clusters of DNA and concentrated macromolecular damage that results in the accumulation of senescent cells (SnCs) known to play a critical role in promoting multimorbidity. Here we demonstrate that human fibroblasts exposed to different forms of space radiation acquire senescence-associated phenotypes including morphological alterations and the accumulation of SA-ßgal+ cells more efficiently than ꝩ-irradiation. Bulk and single cell RNA (scRNAseq) sequencing analysis revealed that space irradiated human fibroblasts up-regulated senescent-like phenotypes to a greater extent than γ-irradiation and enriched pathways associated with chronic activation and adaptation of the integrated stress response and NADPH-coupled redox metabolism. Healthy cells treated with conditioned media from irradiated SnCs manifested pro-inflammatory transcriptional profiles dependent on both radiation and cell type. Finally, treatment with known senotherapeutics demonstrated radiation-specific effects in primary dermal fibroblasts. Our data demonstrate that space radiation differentially induces senescent phenotypes in human cells compared to γ-irradiation that may play a key role in the pathogenic effects of space travel.
2025-12-09 | GSE297560 | GEO
Project description:As Earth’s magnetic field and ozone continue to weaken, space radiation begins pose a significant threat to the health of not only space travelers, but the world’s population. Space radiation and its high-energy and high-charge ions create distinct clusters of DNA and concentrated macromolecular damage that results in the accumulation of senescent cells (SnCs) known to play a critical role in promoting multimorbidity. Here we demonstrate that human fibroblasts exposed to different forms of space radiation acquire senescence-associated phenotypes including morphological alterations and the accumulation of SA-ßgal+ cells more efficiently than ꝩ-irradiation. Bulk and single cell RNA (scRNAseq) sequencing analysis revealed that space irradiated human fibroblasts up-regulated senescent-like phenotypes to a greater extent than γ-irradiation and enriched pathways associated with chronic activation and adaptation of the integrated stress response and NADPH-coupled redox metabolism. Healthy cells treated with conditioned media from irradiated SnCs manifested pro-inflammatory transcriptional profiles dependent on both radiation and cell type. Finally, treatment with known senotherapeutics demonstrated radiation-specific effects in primary dermal fibroblasts. Our data demonstrate that space radiation differentially induces senescent phenotypes in human cells compared to γ-irradiation that may play a key role in the pathogenic effects of space travel.
2025-12-09 | GSE297559 | GEO