Project description:Pollen development is one of the most heat-sensitive developmental stages in a wide range of crops. Our longer-term goal is to understand the mechanism how starch metabolism in maturing pollen grains of the Solanaceae family contributes to maintaining higher pollen quality under heat-stress conditions. The specific aim of the suggested proposal is to characterize N. sylvestris WT and mutant (starch-deficient) transcriptomes during microgametogenesis under ambient and heat-stress conditions. Expression profiles of maturing microspores derived from flower buds at developmental stage of 4 to 2 days before flower opening will be obtained. Pollen was derived from WT and mutant plants exposed to either ambient or heat-stress conditions (exposing the plants to 45oC for 2.5 hours). Keywords: Loop design
Project description:Real-time quantitative PCR analysis of human epithelial cells The activity of Malva sylvestris extract and fractions infected by A. actinomycetemcomitans were investigated using an adapted dual chamber model, oral human epithelial cells were used in the co-culture model. One microgram of RNA was converted in cDNA using RT2 First Strand Kit. 84 genes were analyzed using inflammatory response & Autoimmunity Array RT2 profiler (Qiagen Sabiosciences, Valencia, CA, USA) with buffers supplied by the manufacturer qPCR gene expression profiling. Oral human epithelial cells were infected by A. Actinomycetemcomintans and treated with Malva sylvestris extract and fractions prior to gene expression analysis.
