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Human Brain Small Extracellular Vesicles Contain Selectively-Packaged, Full-Length mRNA [bulk Iso-Seq]

ABSTRACT: Human Brain Small Extracellular Vesicles Contain Selectively-Packaged, Full-Length mRNA [bulk Iso-Seq]

PROVIDER: PRJNA1075068 | ENA |

REPOSITORIES: ENA

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Human Brain Small Extracellular Vesicles Contain Selectively-Packaged, Full-Length mRNA

Project description:Human Brain Small Extracellular Vesicles Contain Selectively-Packaged, Full-Length mRNA

| PRJNA1019233 | ENA

Human Brain Small Extracellular Vesicles Contain Selectively-Packaged, Full-Length mRNA [bulk Iso-Seq]

Project description:Brain cells release and take up small extracellular vesicles (sEVs) containing bioactive nucleic acids. sEV exchange is hypothesized to contribute to stereotyped spread of neuropathological changes in the diseased brain. We assessed mRNA from sEVs of non-diseased (ND) and Alzheimer’s disease (AD) human postmortem brains, using short- and long-read sequencing. sEV transcriptomes were distinct from bulk tissue, showing enrichment for multiple genes including mRNAs encoding ribosomal proteins and L1Hs transposable elements. AD versus ND sEVs showed enrichment of inflammation-related and depletion of synaptic signaling mRNAs. sEV mRNA from cultured murine primary neurons, astrocytes, or microglia showed similarities with human brain sEVs and revealed cell-type specific packaging. Nearly 80% of human brain sEV transcripts sequenced using long-read sequencing were full-length. Motif analyses of sEV-enriched full-length isoforms revealed RNA-binding proteins that may be associated with sEV loading. Collectively, we show that mRNA in brain sEVs is selectively-packaged and altered by disease state.

2024-03-14 | GSE255462 | GEO

Human Brain Small Extracellular Vesicles Contain Selectively-Packaged, Full-Length mRNA

Project description:Brain cells release and take up small extracellular vesicles (sEVs) containing bioactive nucleic acids. sEV exchange is hypothesized to contribute to stereotyped spread of neuropathological changes in the diseased brain. We assessed mRNA from sEVs of non-diseased (ND) and Alzheimer’s disease (AD) human postmortem brain, using short- and long-read sequencing. sEV transcriptomes were distinct from bulk tissue, showing enrichment for multiple genes including mRNAs encoding ribosomal proteins and L1Hs transposable elements. AD versus ND sEVs showed enrichment of inflammation-related and depletion of synaptic signaling mRNAs. sEV mRNA from cultured murine primary neurons, astrocytes, or microglia showed similarities with human brain sEVs and revealed cell-type specific packaging. Nearly 80% of human brain sEV transcripts sequenced using long-read sequencing were full-length. Motif analyses of sEV-enriched full-length isoforms revealed RNA-binding proteins that may be associated with sEV loading. Collectively, we show that mRNA in brain sEVs is selectively-packaged and altered by disease state.

2024-03-14 | GSE243611 | GEO

Assembly of full-length macaque gene models

Project description:We reported an atlas of de novo-defined, full-length macaque gene models on the basis of single molecule long-read transcriptome sequencing (Iso-seq).

2021-06-01 | GSE132413 | GEO

packaged drinking water

Project description:packaged drinking water Metagenome

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Evaluation of the effects of bowel cleansing of Jing Chang Pre-packaged food

Project description:Interventions: A:JingChang Pre-packaged Product No.1 (4g Konjac Powder);G:JingChang Pre-packaged Product No.2 (2g Konjac Powder);F:JingChang Pre-packaged Product No.3 (1g Konjac Powder);D:Give competing formulations currently available on the market;E:Self prepared diet Primary outcome(s): Clarity of stools after bowel cleansing Study Design: Parallel

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Cisplatin-induced decrease of mRNA length reveals functional and translated intronic-polyadenylation isoforms in the annotated 5’-untranslated region of genes [ISO-seq]

Project description:We explored the regulation of intronic polyadenylation isoforms by cisplatin, and its impact on the translatome. This was done in part by 3'-seq (see GSE119978) and Iso-Seq (this data set).

2022-07-31 | GSE180918 | GEO

Resistance to hop iso-α-acids in yeast involves active export and vacuolar sequestration

Project description:The hop plant, Humulus lupulus L., contains an exceptionally high content of secondary metabolites, the hop iso-α-acids, which possess a range of beneficial properties including antiseptic action. Studies performed on the mode of action of hop iso-α-acids have hitherto been restricted to lactic acid bacteria. The present study investigates molecular mechanisms of hop iso-α-acid resistance in the model eukaryote Saccharomyces cerevisiae. Growth inhibition occurred at concentrations of hop iso-α-acids that were an order of magnitude higher than those found with hop-tolerant prokaryotes. Chemostat-based transcriptome analysis and phenotype screening of the S. cerevisiae haploid gene deletion collection were used as complementary methods to screen for genes involved in hop iso-α-acids detoxification and tolerance. Further analysis of deletion mutants confirmed that yeast tolerance to hop iso-α-acids involves two major processes: active export of iso-α-acids across the plasma membrane and active proton pumping into the vacuole by the V-ATPase to enable vacuolar sequestration of iso-α-acids. Furthermore, iso-α-acids were shown to affect cellular metal homeostasis by acting as strong zinc and iron chelator.

2009-11-13 | GSE15094 | GEO

Expression data from isorhamnetin (Iso)-treated human amnion epithelial stem cells (hAESCs)

Project description:Gene expression profiling reveals a potential role of Iso towards hepatic differentiation of hAESCs. hAESCs were isolated from discarded term placenta and were treated with 20 μM Iso for 10 days. Microarray gene expression profiling was conducted for biological replicates of Iso-treated hAESCs on day 10 and untreated control cells on day 0, and day 10.

2020-06-25 | GSE153149 | GEO

Vacuum packaged beef and lamb

Project description:Vacuum packaged beef and lamb Targeted loci

| PRJNA580325 | ENA