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ABSTRACT: Australian Amphibian and Reptile Genomics (ARG)

PROVIDER: PRJNA1075730 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR24957355_1.fastq.gz	Fastqsanger.gz
	SRR24957355_2.fastq.gz	Fastqsanger.gz
	SRR24957356_1.fastq.gz	Fastqsanger.gz
	SRR24957357_1.fastq.gz	Fastqsanger.gz

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Project description:L-Arginine (L-Arg) is the substrate for both inducible nitric oxide synthase and arginase, which are upregulated in human IBD and in mouse colitis models. We have found that L-Arg supplementation enhances wound restitution in vitro, and improves the clinical parameters of weight loss, survival, and colon weight/length, in dextran sulfate sodium (DSS) induced murine colitis. Our aim was to further identify the potential mechanisms underlying the clinical benefit of L-Arg supplementation. 12 Total samples were analyzed, 3 samples from each of 4 groups. We generated the following pairwise comparisons: Ctrl vs Ctrl + L-Arg; Ctrl vs DSS; DSS vs DSS + L-Arg; Ctrl + L-Arg vs DSS, Ctrl + L-Arg vs DSS + L-Arg. Genes with a p-value < 0.01 and a fold-change ≥2 were selected. To identify genes that were altered in response to L-Arg, we performed the following multiple sample comparisons using a p-value < 0.01 and a fold-change ≥2: Ctrl vs DSS vs DSS + L-Arg

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