Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA expression in Monocytes from thrombocytopenic mice

ABSTRACT: RNA expression in Monocytes from thrombocytopenic mice

PROVIDER: PRJNA1078062 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28009955.fastq.gz	Fastqsanger.gz
	SRR28009956.fastq.gz	Fastqsanger.gz
	SRR28009957.fastq.gz	Fastqsanger.gz
	SRR28009958.fastq.gz	Fastqsanger.gz
	SRR28009959.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Histone modifications of Monocytes in the context of thrombocytopenia

Project description:We sought to define changes in open and closed chromatin in monocytes isolated from control and thrombocytopenic mice

2024-04-12 | GSE256085 | GEO

Staphylococcus aureus Mediated Tolerance Induction in Human Monocytes is accompanied with Specific Alterations in Membrane Receptor Expression

Project description:Exposure of human monocytes to lipopolysaccharide (LPS) or other pathogen-associated molecular pattern (PAMPs) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance (ET). The tolerant state of monocytes is accompanied by cell surface and other glycoprotein expression changes induced by the activation of Toll-like receptors (TLRs). In this study, we aimed to characterize the cellular state of human monocytes stimulated with Gram-positive Staphylococcus aureus and TLR2 ligands. We analyzed gene expression changes induced by S. aureus after 2 and 24 hours by amplicon sequencing (RNA-AmpliSeq) and compared the pro-inflammatory response after 2 hours of stimulation to the to the response in re-stimulation experiments after the first stimulus. In parallel, glycoprotein expression changes in human monocytes after 24 hours of S. aureus stimulation were analyzed by proteomics and compared to stimulation experiments with TLR2 ligands Malp-2 and Pam3Cys and TLR4 ligand LPS. The results demonstrate that monocytes stimulated with S. aureus and TLR ligands entered the tolerant cell state after activation. Compared to TLR agonist mediated activation and tolerization of monocytes, glycoprotein expression changes induced by S. aureus stimulation revealed significant differences in receptor expression profiles. We report a glycoprotein expression profile characteristic for PAMP and S. aureus tolerized human monocytes. Finally, we analyzed peripheral blood monocytes of patients with S. aureus bloodstream infection for inflammatory responses in vitro and for their glycoprotein expression profiles. RNA-AmpliSeq data from patient-derived monocytes demonstrated that the cells were pro-inflammatory responsive to S. aureus stimulation and expressed higher level of CD44 mRNA, while other markers of the tolerant cell state were not detected.

2023-05-10 | PXD036036 | Pride

Gene expression profile of inflammatory monocytes in cachectic blood cancer mice [bulk RNA-seq]

Project description:Gene expression profile of inflammatory monocytes in cachectic blood cancer mice [bulk RNA-seq]

| PRJNA863303 | ENA

RNA-Seq analysis of CD115- monocytes and CD115+ monocytes from naïve and tumor-bearing mice

Project description:RNA-Seq analysis of CD115- monocytes and CD115+ monocytes from naïve and tumor-bearing mice

| PRJNA817820 | ENA

RNA-Seq analysis of CD115- monocytes and CD115+ monocytes from naïve and tumor-bearing mice

Project description:Purpose: The goals of this study are to compare CD115- monocytes to CD115+ monocytes from naïve and tumor-bearing mice (EL4) with high-throughput data analysis. Methods: CD115- monocytes and CD115+ monocytes were sorted by flow cytomentry separatly. Total RNA was extracted from cells using the RNeasy Mini Kit (QIAGEN, Hilden, Germany) and residual DNA was removed with a DNA-free Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. Samples were loaded onto a 1% agarose gel before electrophoresis. DNA-free conditions were confirmed by SYBR Gold staining (Invitrogen). The quantity and the quality of RNA was evaluated by Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific) and bioanalyzer (Agilent 2100; Agilent Technologies, Santa Clara, CA, USA). RNA-Seq libraries were prepared with 1 μg of total RNA from two biological replicates of each group using TruSeq Stranded mRNA LT Sample Prep Kit and analyzed by Illumina Sequencer (both from Illumina, San Diego, CA, USA). Results: We found 1,723 differetially expressed genes (DEGs) between CD115- monocytes and CD115+ monocytes (cutoff value was set at 2-fold). DEGs of interest are invoved in granulocyte, transcription factors, mononuclear phagocytes, and angiogenesis. Conclusions: Our study represents the first detailed DEGs analysis of CD115- monocytes and CD115+ monocytes from naïve and tumor-bearing mice. Our results show that CD115- monocytes may have a closer relationship with neutrophils or PMN-MDSCs than the CD115+ monocytes. Also, our overall transcriptomic analysis further suggests that CD115- M-MDSCs and monocytes are separate subsets of monocytic cells.

2022-03-22 | GSE199008 | GEO

RNA sequencing human monocytes

Project description:RNA sequencing human monocytes

| PRJNA533229 | ENA

Assessment Of Long Noncoding RNA CCAT1 In Colorectal Cancer Patients

Project description:1. Evaluate the diagnostic value of long noncoding RNA (CCAT1) expression by RT-PCR in peripheral blood in colorectal cancer patients versus normal healthy control personal. 2. Evaluate the clinical utility of detecting long noncoding RNA (CCAT1) expression in diagnosis of colorectal cancer patients & its relation to tumor staging. 3. Evaluate the clinical utility of detecting long noncoding RNA (CCAT1) expression in precancerous colorectal diseases. 4. Compare long noncoding RNA (CCAT1) expression with traditional marker; carcinoembryonic antigen (CEA) and Carbohydrate antigen 19-9 (CA19-9) in diagnosis of colorectal cancer.

| 2330122 | ecrin-mdr-crc

Gene expression analysis of neutrophils, Ym1+Ly6Chi monocytes and Ym1-Ly6Chi monocytes

Project description:mRNA expression profiles between Ym1+Ly6Chi monocytes and Ym1-Ly6Chi monocytes from LPS-treated mice were analyzed by RNA-sequencing

2021-07-02 | GSE118032 | GEO

RNA-seq of inflammatory monocytes, patrolling monocytes and neutrophils in wild type and ABIN1[D485N] mice

Project description:RNA-seq of inflammatory monocytes, patrolling monocytes and neutrophils in wild type and ABIN1[D485N] mice

| PRJEB33670 | ENA

RNA expression in Monocytes from thrombocytopenic mice

Project description:We sought to define changes in gene expression in monocytes in the context of thrombocytopenia

2024-04-12 | GSE256083 | GEO