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ABSTRACT: Skin microbiota of pregnant women

PROVIDER: PRJNA1078078 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR28007217_1.fastq.gz	Fastqsanger.gz
	SRR28007217_2.fastq.gz	Fastqsanger.gz
	SRR28007218_1.fastq.gz	Fastqsanger.gz
	SRR28007218_2.fastq.gz	Fastqsanger.gz
	SRR28007219_1.fastq.gz	Fastqsanger.gz

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Project description:Background: Knowledge about extracellular vesicles (EV) and their molecular cargo in gestational parasitic infections, particularly by Plasmodium and soil-transmitted helminths (STH), is almost non-existent. Objective: To perform isolation and molecular characterization of plasma-derived EVs from Colombian pregnant women and compare quantity, size, concentration and protein cargo of those EVs according to the infectious status. Methodology: Five study groups were formed: 1), Pregnant women with Plasmodium infection. 2), Pregnant women with STH infection. 3), Pregnant women with coinfection Plasmodium and STH. 4), Pregnant women without infection with Plasmodium nor STH. 5), Non-pregnant women without infection with Plasmodium nor STH. Plasma-derived EVs were isolated by size exclusion chromatography (SEC) and fractions containing EVs identified by a bead-based flow cytometric assay for tetraspanin CD9; the size and concentration of EVs were quantified by nanoparticle tracking analysis, and proteins associated with EVs were identified by liquid chromatography-mass spectrometry (LC-MS) in a pool of samples per study group.

2024-11-26 | PXD051270 | Pride

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