Solanum tuberosum
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ABSTRACT:
PROVIDER: PRJNA107939 | ENA |
REPOSITORIES: ENA
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2016-12-14 | PXD004814 | Pride
Project description:PVY-NTN elicits diverse gene expression response in different potato genotypes in the first 12 hours after inoculation
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Project description:Many virus diseases of economic importance to agriculture result from mixtures of different pathogens invading the host at a given time. This contrasts with the relatively scarce studies available on the molecular events associated with virus-host interactions in mixed infections. In comparison to single infections, co-infection of Nicotiana benthamiana with Potato virus X (PVX) and Potato virus Y (PVY) resulted in increased systemic symptoms (synergism) that led to necrosis of the newly emerging leaves, and the plant death. A comparative transcriptional analysis was undertaken to identify quantitative and qualitative differences in gene expression during this synergistic infection, and to correlate these changes with the severe symptoms it caused. Global transcription profiles of doubly-infected leaves were compared with those from singly-infected leaves using gene ontology enrichment analysis and metabolic pathway annotator software. Functional gene categories altered by the double infection comprise suites of genes regulated coordinately, which are associated with chloroplast functions (down-regulated), protein synthesis and degradation (up-regulated), carbohydrate metabolism (up-regulated), and response to biotic stimulus and stress (up-regulated). The expression of reactive oxygen species-generating enzymes as well as several mitogen-activated protein kinases, were also significantly induced. Accordingly, synergistic infection induced a severe oxidative stress in N. benthamiana leaves, as judged by increases in lipid peroxidation, and by the generation of superoxide radicals in chloroplasts, which correlated with the misregulation of antioxidative genes in microarray data. Interestingly, expression of genes encoding oxylipin biosynthesis was uniquely up-regulated by the synergistic infection. Virus-induced gene silencing of alfa-dioxygenase1 delayed cell death during PVX-PVY infection. Using mock inoculated leaf tissue as a reference, we compare the gene expression profiles of Nicotiana benthamiana plants infected with one of two viruses, Potato virus X (PVX) or Potato virus Y (PVY), or the combination PVX plus PVY. 3 biological replicates per treatment were independently grown and haversted.
2010-05-16 | E-GEOD-15538 | biostudies-arrayexpress
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2007-09-01 | GSE8255 | GEO
Project description:Identification of Gene Expression Patterns During Somatic Embryogenesis in Potato
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Project description:Pti5 is an ERF transcription factor that was differentially expressed in PVY-infected potato plants. The purpose of generating this dataset was to identify genes with perturbed expression due to silencing the Pti5 gene. Grant ID: P4-0165 Slovenian Research Agency ARRS Biotechnology and Plant Systems Biology Grant ID: J4-7636 Slovenian Research Agency ARRS Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato Grant ID: J4-1777 Slovenian Research Agency ARRS Unraveling mechanisms of effectiveness and specificity in potato immune signaling through innovative data acquisition and modeling
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