Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Locolization of circRNA with DIS3 WT or D146N mutant in sucrose density gradient centrifugation assay

ABSTRACT: Locolization of circRNA with DIS3 WT or D146N mutant in sucrose density gradient centrifugation assay

PROVIDER: PRJNA1079793 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Locolization of circRNA with DIS3 WT or D146N mutant in sucrose density gradient centrifugation assay

Project description:To investigate the locolization between DIS3 and circRNA in fractions form sucrose density gradient centrifugation (SDGC) assay for 293T cells

2025-02-16 | GSE256498 | GEO

Effect of DIS3 knockdown on circRNA expression

Project description:To investigate the effect of DIS3 KD on circRNA expression in 293T cell line

2025-02-16 | GSE256341 | GEO

Effect of DIS3 knockdown on circRNA expression

Project description:Effect of DIS3 knockdown on circRNA expression

| PRJNA1079231 | ENA

Ribosome profiling on polysomes extracted via sucrose gradient and IP

Project description:Technical feasibility study to determine data quality and reproducibilty. Ribosome profiling was performed by either sucrose gradient (SG) or immunoprecipitation (IP).

2016-02-17 | GSE74511 | GEO

Escherichia coli sucrose gradient fractionation

Project description:We studied localization of E. coli membrane proteome using sucrose gradient fractionation combined with quantitative MS.

2020-02-19 | PXD016403 | Pride

Ribosome profiling on polysomes extracted via sucrose gradient and IP

Project description:Technical feasibility study to determine data quality and reproducibilty. Ribosome profiling was performed by either sucrose gradient (SG) or immunoprecipitation (IP). 1 sucrose gradient sample, 2 immunoprecipitation samples in two concentrations of beads

2016-02-17 | E-GEOD-74511 | biostudies-arrayexpress

DIS3 ablation in spermatocytes causes defective spermatogenesis in mice

Project description:Conditional inactivation of DIS3 in spermatocytes impairs meiotic progression and causes defective spermatogenesis in mice. scRNA-seq analysis indicates that DIS3 mutation significantly impairs spermatocytes meiotic progression and dysregulates transcriptome in spermatocytes. We show that DIS3 ribonuclease plays critical roles in male meiotic progression and spermatogenesis in mice.

2024-12-13 | GSE274927 | GEO

Human DIS3 shapes the RNA polymerase II transcriptome degrading variety of unwanted transcripts.

Project description:Human DIS3 is a nuclear, catalytic subunit of the exosome complex containing exonucleolytic and endonucleolytic active domains. To identify DIS3 targets genome-wide we conducted comprehensive transcriptomic analysis of HEK293 cells producing mutated DIS3 versions and Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) experiments. Pervasive transcription products like Promoter Upstream Transcripts (PROMPTs) accumulated robustly in catalytic DIS3 mutants, representing ~8% of PAR-CLIP reads. Importantly, RNAs originating from unannotated genomic regions increased ~2.5 times in double DIS3 mutants, covering ~70% of genome and allowing for discovery of thousands of novel transcripts. The first intron of many pre-mRNAs accumulated in DIS3 mutants indicating a widespread premature RNA polymerase II termination. The short form of NEAT1 lincRNA was overexpressed in DIS3 mutants, leading to increased number of paraspeckles. Moreover, there was a global deregulation of mRNAs in DIS3 double mutant. Finally, snoRNA precursors accumulated, which correlated with a strong PAR-CLIP signal indicating that DIS3 but not RRP6 is a main snoRNA processing enzyme. In aggregate, we demonstrate that DIS3 is a major nucleoplasmic activity responsible for shaping the human transcriptome.

2015-08-20 | GSE64332 | GEO

Rumen bacteria diversity in a sucrose density gradient

Project description:Study of rumen bacterial diversity using a sucrose density gradient to separate it by size and density. In this way, it is possible to enriched in the fractions of the gradient, the low abundant bacteria in the rumen and performs shotgun sequencing which will facilitate to know the function of these bacterial groups.

| PRJEB42578 | ENA

DIS3 ribonuclease is required for the maintenance of spermatogenic lineage in mice

Project description:Spermatogonial stem cells (SSCs) self-renewal and differentiation are the foundation for continious spermatognenesis in mice. Here, we investigate the essential role of DIS3 in maintaining SSC homeostasis and facilitating germ cell differentiation to ensure male fertility. Conditional inactivation of DIS3 in male germ cells have severely impaired SSC self-renewal and differentiation, which results in the failure of spermatogenesis associated with a Sertoli cell-only syndrome and adult sterility. RNA-seq analysis reveales that Dis3 deficiency abolishes its nucleolytic activity and causes significant dysregulation of the expression of transcripts in Dis3 mutant testes. We have also found that the pervasive transcription products described previously, such as Promoter Upstream Transcripts (PROMPTs), accumulate robustly upon DIS3 dysfunction in Dis3 cKO testes. In addition, scRNA-seq analysis indicates that DIS3 mutation significantly impairs germline stem cell development that blocks stem cell proliferation and differentiation. Overall, we show that DIS3 ribonuclease plays a critical role in the maintenance of spermatogenic lineage during spermatogenesis in mice.

2020-08-20 | GSE156505 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data