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Escherichia coli sucrose gradient fractionation

ABSTRACT: We studied localization of E. coli membrane proteome using sucrose gradient fractionation combined with quantitative MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Escherichia Coli

SUBMITTER: Frank Stein

LAB HEAD: Athanasios Typas

PROVIDER: PXD016403 | Pride | 2020-02-19

REPOSITORIES: Pride

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			Action	DRS
	F081751.dat	Other
	F081753.dat	Other
	F081758.dat	Other
	F081761.dat	Other
	F081767.dat	Other

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Publications

Systematic Localization of Escherichia coli Membrane Proteins.

Sueki Anna A Stein Frank F Savitski Mikhail M MM Selkrig Joel J Typas Athanasios A

mSystems 20200303 2

The molecular architecture and function of the Gram-negative bacterial cell envelope are dictated by protein composition and localization. Proteins that localize to the inner membranes (IM) and outer membranes (OM) of Gram-negative bacteria play critical and distinct roles in cellular physiology; however, approaches to systematically interrogate their distribution across both membranes and the soluble cell fraction are lacking. Here, we employed multiplexed quantitative mass spectrometry using t ...[more]

PMID: 32127419

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