Project description:We report here that gentic methylation in the male germline, from meiocytes to sperm, is established by siRNAs transcribed from transposons with imperfect sequence homology. These siRNAs are synthesized by meiocyte nurse cells (tapetum) via activity of the chromatin remodeler CLASSY3, which is specifically expressed in tapetal cells. Plants that produce siRNAs only in the tapetum have broadly normal DNA methylation of the entire germline. Finally, we also report that these nurse cell-derived siRNAs (niRNAs) silence germline transposons, thereby safeguarding genome integrity. Our results reveal the crucial role of tapetal niRNAs in germline methylation reprogramming, which is remarkably analogous to piRNA-mediated reprogramming in animal germlines.
Project description:The aim of this work was to extend the previous gene expression analysis ofM- M- nurse and forager gene expression, but using the Apis oligo-array designed from the recently sequenced genome.M- M- This study included 28000 features which included ~11000 predicted genes.M- M- The intial study was done using a microarray design based on PCR products of 5500 features derived from an expressed sequence tag analysis of a brain cDNA library.M- M- The variables studied were age (young and old), cast (nurse and forager) and differential gene expression.
Project description:Monocytes and matching culture-derived nurse-like cells were compared using Illumina's EPIC/850k arrays Monocytes isolated using MACS and NLCs isolated using cytometry sorting as CD14+/CD19-
Project description:The study of the roles of macrophages in the microenvironment of cancer cells (tumor-associated macrophages, TAM) has gained deep insight over the recent years. Here, we describe gene expression profile of chronic lymphocytic leukemia (CLL)-associated macrophages, also called nurse-like cells (NLC), derived from in vitro co-cultures system. We define these NLC as M2-oriented, TAM-like specific subset of macrophages, with very few inter-individual variations in gene expression profiles.
Project description:LC-MS/MS proteomics was used to identify immune proteins in the plasma of the nurse shark (Ginglymostoma cirratum), using a de novo multi-tissue transcriptome generated for this species. LC-MS/MS was then used to assess the host response to immunization with human serum albumin (HSA) and Complete Freund’s Adjuvant (CFA).
