Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ovo KD RNA-Seq]

ABSTRACT: Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ovo KD RNA-Seq]

PROVIDER: PRJNA1081377 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28122445_1.fastq.gz	Fastqsanger.gz
	SRR28122445_2.fastq.gz	Fastqsanger.gz
	SRR28122446_1.fastq.gz	Fastqsanger.gz
	SRR28122446_2.fastq.gz	Fastqsanger.gz
	SRR28122447_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary

Project description:The gene-regulatory mechanisms controlling the expression of the germline PIWI-interacting RNA (piRNA) pathway components within the gonads of metazoan species remain largely unexplored. In contrast to the male germline piRNA pathway, which in mice is known to be activated by the testis-specific transcription factor A-MYB, the nature of the ovary-specific gene-regulatory network driving the female germline piRNA pathway remains a mystery. Here, using Drosophila as a model, we combined multiple genomics approaches to reveal the transcription factor Ovo as regulator of the germline piRNA pathway in ovarian germ cells. Ectopic expression of Ovo in ovarian somatic cells activates germline piRNA pathway components, including the ping-pong factors Aubergine, Argonaute-3, and Vasa, leading to assembly of perinuclear cellular structures resembling nuage bodies of germ cells. We found that in ovarian somatic cells, transcription of ovo is repressed by l(3)mbt, thus preventing expression of germline piRNA pathway genes in the soma. Cross-species ChIP-seq and motif analyses demonstrate that Ovo is binding to genomic CCGTTA motifs within the promoters of germline piRNA pathway genes, suggesting a regulation by Ovo in ovaries analogous to that of A-MYB in testes. Our results also show consistent engagement of the Ovo transcription factor family at ovarian piRNA clusters across metazoan species, reflecting a deep evolutionary conservation of this regulatory paradigm from insects to humans.

2024-12-23 | GSE233246 | GEO

Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ovo KD RNA-Seq]

Project description:The gene-regulatory mechanisms behind the female germline piRNA pathway in ovaries of metazoan species remain largely unexplored. Here, using the Drosophila model and combinations of ChIP-seq, RNA-seq, ATAC-seq, and single-cell approaches, we reveal the transcription factors that regulate the female germline piRNA pathway in animal ovaries.

2024-12-23 | GSE259368 | GEO

Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary

Project description:Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary

| PRJNA975551 | ENA

Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [RNA-seq]

Project description:The gene-regulatory mechanisms behind the female germline piRNA pathway in ovaries of metazoan species remain largely unexplored. Here, using the Drosophila model and combinations of RNA-seq, ATAC-seq, and single-cell approaches, we reveal the transcription factors that regulate the female germline piRNA pathway in animal ovaries.

2024-12-23 | GSE233245 | GEO

Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ATAC-seq]

Project description:The gene-regulatory mechanisms behind the female germline piRNA pathway in ovaries of metazoan species remain largely unexplored. Here, using the Drosophila model and combinations of RNA-seq, ATAC-seq, and single-cell approaches, we reveal the transcription factors that regulate the female germline piRNA pathway in animal ovaries.

2024-12-23 | GSE233241 | GEO

Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ChIP-Seq]

2024-12-23 | GSE259366 | GEO

Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ChIP-Seq]

Project description:Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ChIP-Seq]

| PRJNA1081372 | ENA

Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary

Project description:Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary

| PRJNA116399 | ENA

Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ATAC-seq]

Project description:Transcriptional regulation of the germline piRNA pathway in the Drosophila ovary [ATAC-seq]

| PRJNA975540 | ENA