Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of depletion Mina53 on the expression of genes in NCM460 cells [RNA-seq]

ABSTRACT: Effects of depletion Mina53 on the expression of genes in NCM460 cells [RNA-seq]

PROVIDER: PRJNA1082891 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Effects of depletion Mina53 on the expression of genes in NCM460 cells [RNA-seq]

Project description:In order to investigate the effect of Mina53 in the epithelial cell line NCM460 cells, we depleted the expression of Mina53 in NCM460 cells and performed RNA-seq to investigate genes regulated by Mina53 and thus find the signaling pathways Mina53 participated in NCM460 cells.

2025-02-01 | GSE260650 | GEO

Decoding the effect of Mina53 in the distribution of H3K9me3 in NCM460 cells

Project description:In oder to investigate genes directly regulated by Mina53, We performed CUT&TAG assay to dectected changes of H3K9me3 level on the whole genome in Mina53-KD NCM460 cells compared with the control group.

2025-02-01 | GSE260664 | GEO

Decoding the effect of Mina53 in the distribution of H3K9me3 in NCM460 cells

Project description:Decoding the effect of Mina53 in the distribution of H3K9me3 in NCM460 cells

| PRJNA1082650 | ENA

RNA-seq of depletion Mina53 in HCT116 p53+/+ cells and HCT116 p53-/- cells

Project description:We report the signaling pathway of Mina53 by depleting Mina53 in HCT116 p53+/+ cells and HCT116 p53-/- cells based on RNA-sequencing.

2024-12-01 | GSE214730 | GEO

Mina53 regulates the expression of downstream genes in NSPC cells by its arginine demethylase activity on H4R3me2a

Project description:In order to investigate the role of Mina53 in the NSPC proliferation and differentiation, we performed RNA-seq using Mina53-KO NSPCs and wild-type NSPCs; we performed CUT-TAG using anti-H4R3me2a antibody in Mina53-KO NSPCs and wild-type NSPCs.

2024-11-14 | GSE275270 | GEO

Mina53 demethylates histone 4 arginine 3 asymmetric di-methylation to regulate neural stem/progenitor cell identity

Project description:Arginine methylation of histones plays a critical role in regulating gene expression. The writers (methyltransferases) and readers of methylarginine marks are well-known, but the erasers－arginine demethylases－remain mysterious. Here we identify Myc-induced nuclear antigen 53 (Mina53), a jumonji C domain containing protein, as an arginine demethylase for removing asymmetric di-methylation at arginine 3 of histone H4 (H4R3me2a). Using photoaffinity capture method, we first identified Mina53 as an interactor of H4R3me2a. Biochemical assays in vitro and in cells characterized the arginine demethylation activity of Mina53. Molecular dynamics simulations provide further atomic-level evidence that Mina53 acts on H4R3me2a. In a transgenic mouse model, specific Mina53 deletion in neural stem/progenitor cells prevented H4R3me2a demethylation at distinct genes clusters, dysregulating genes important for neural stem/progenitor cell proliferation and differentiation, and consequently impairing the cognitive function of mice. Collectively, we identify Mina53 as a bona fide H4R3me2a eraser, expanding the understanding of epigenetic gene regulation.

2024-10-22 | PXD045247 | Pride

Mina53 regulates the neural stem cell pool by acting as an arginine demethylase

Project description:To investigate the function Mina53 in the regulation of neural stem cells proliferation and differentiation, we collect neural stem cells which Mina53 has been knocked down by shRNA.

2024-05-20 | GSE242238 | GEO

Mina53 regulates the neural stem cell pool by acting as an argnine demethlysae

2024-05-20 | GSE232829 | GEO

RNA-seq of depletion Mina53 in HCT116 p53+/+ cells and HCT116 p53-/- cells

Project description:RNA-seq of depletion Mina53 in HCT116 p53+/+ cells and HCT116 p53-/- cells

| PRJNA886961 | ENA

Effects of Id1 depletion in TAMs on the genes expression of MC38 cells.

Project description:Effects of Id1 depletion in TAMs on the genes expression of MC38 cells.

| PRJNA827027 | ENA